Brown Adipose Tissue: Methods and Protocols

ISBN-13: 9781071620892 / Angielski / Miękka / 2022 / 309 str.

David A. Guertin; Christian Wolfrum

Opis

This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and 'omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Brown Adipose Tissue: Methods and Protocols provides perspectives and detailed protocols for the benefit of both new BAT researchers looking for guidance as well as seasoned researchers who would like to expand their toolkits. 

Chapter 12 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

1. Brown Adipose Tissue: A Short Historical Perspective

            Paul Trayhurn

 

2. Brown Fat Anatomy in Humans and Rodents

            Georgia Colleluori, Jessica Perugini, Angelica Di Vincenzo, Martina Senzacqua, Antonio Giordano, and Saverio Cinti

 

3. Analysis of Thermogenesis Experiments with CalR

            Marissa D. Cortopassi, Deepti Ramachandran, William B. Rubio, Daniel Hochbaum, Bernardo L. Sabatini, and Alexander S. Banks

 

4. A Clearing Method for Three-Dimensional Imaging of Adipose Tissue

            Zeran Lin, Jingyi Chi, and Paul Cohen

 

5. Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron

            Kong Y. Chen, Robert J. Brychta, Nikita S. Israni, Alex Jiang, Hannah J. Lea, Taylor N. Lentz, Annie E. Pierce, and Aaron M. Cypess

 

6. Fluorescent and Luminescent Methods to Detect Lipolysis

            Emilio P. Mottillo and James G. Granneman

 

7. Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice

            Markus Heine, Carlotta Corban, and Joerg Heeren

 

8. Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes

            Su Myung Jung, Johnny Le, Will G. Doxsey, John A. Haley, Grace Park, David A. Guertin, and Cholsoon Jang

 

9. Activation of UCP1-Independent Ca²⁺ Cycling Thermogenesis by Wireless Optogenetics

            Kenji Ikeda, Kazuki Tajima, Yuji Tanabe, Ada S.Y. Poon, and Shingo Kajimura

 

10. Measurement of Futile Creatine Cycling Using Respirometry

            Janane F. Rahbani, Edward T. Chouchani, Bruce M. Spiegelman, and Lawrence Kazak

 

11. Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes

            Anne Loft, Maja Worm Andersen, Jesper Grud Skat Madsen, and Susanne Mandrup

 

12. Diet-Induced Thermogenesis: Principles and Pitfalls

            Jan Nedergaard and Barbara Cannon

 

13. Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice

            Rajib Mukherjee and Joan Sanchez-Gurmaches

 

14. Isolation and Characterization of Human Brown Adipocytes

            Camilla Scheele, Tora Ida Henriksen, and Søren Nielsen

 

15. Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The ‘Goldilocks Solution’

            Min Li and John R. Speakman

 

16. Signaling Lipidomic Analysis of Thermogenic Adipocytes

            Sean D. Kodani, Valerie Bussberg, Niven R. Narain, Michael A. Kiebish, and Yu-Hua Tseng

 

17. Blood Sampling for Arteriovenous Difference Measurements Across Interscapular Brown Adipose Tissue in Rat

            Alberto Mestres-Arenas, Montserrat Cairó, Marion Peyrou, and Francesc Villarroya

 

18. In Vivo Imaging of Brown Adipose Tissue in Humans: FDG-PET/CT and Beyond

            Kirsi A. Virtanen

 

19. Analysis of Single Cell/Nucleus Transcriptome Data in Adipose Tissue

            Wenfei Sun