Bioluminescence: Methods and Protocols, Volume 2

ISBN-13: 9781071624722 / Angielski / Twarda / 2022

Kim, Sung-Bae

Opis

This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 2 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Spis treści

Part I: Heterogeneous Conjugates

 

1. Quantitative Assessment of the Efficacy of Near-Infrared Photoimmunotherapy with Bioluminescence Imaging

            Ryuhei Okada, Aki Furusawa, Peter L. Choyke, and Hisataka Kobayashi

 

2. Evaluation of the Efficacy of Saracatinib-Loaded Nanoparticles in Lymphatic Metastases of HNSCC with the Aid of Bioluminescence Imaging

            Liwei Lang and Yong Teng

 

3. Reactive Oxygen Species-Responsive and Self-Illuminating Nanoparticles for Inflammation and Tumor Imaging

            Xiaoqiu Xu, Qi Li, and Jianxiang Zhang

 

4. Antibacterial Activity Evaluation of ZnO, CuO, and TiO₂ Nanoparticles in Solution and Thin Films

            Christine Mielcarek, Rania Dadi, Anne Roynette, Alex Lemarchand, Andrei Kanaev, Karim Senni, Mamadou Traore, and Rabah Azouani

 

5. BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate

            Setsuko Tsuboi and Takashi Jin

 

6. Polyhistidine-Tag-Enabled Conjugation of Quantum Dots and Enzymes to DNA Nanostructures

            Christopher M. Green, Divita Mathur, Kimihiro Susumu, Eunkeu Oh, Igor L. Medintz, and Sebastián A. Díaz

 

7. Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes

            Roxanne Castillo, Di Wu, Zheng Cao, Ran Yan, Kalea Fajardo, Jie Ren, Yunfeng Lu, and Jing Wen

 

Part II: Protein Fragment-Complementation Assays

 

8. Quantitative Imaging of Retinoic Acid Activities in Living Mammalian Cells

            Sung-Bae Kim, Rika Fujii, and Ramasamy Paulmurugan

 

9. Bioluminescence-Based Complementation Assay to Correlate Conformational Changes in Membrane-Bound Complexes with Enzymatic Function

            Sharon O’Neill and Ulla G. Knaus

 

10. The NanoBiT-Based Homogenous Ligand-Receptor Binding Assay

            Ya-Li Liu and Zhan-Yun Guo

 

11. Monitoring Hippo Signaling Pathway Activity Using a Luciferase-Based Large Tumor Suppressor (LATS) Biosensor

            Alexander Pipchuk and Xiaolong Yang

 

Part III: BRET-Based Imaging

 

12. Screening of Protein–Protein Interaction Modulators Using BRET‑Based Technology

            Lucia R. Fernández, Jesica Mild, and Martin M. Edreira

 

13. TRUPATH: An Open-Source Biosensor Platform for Interrogating the GPCR Transducerome

            Jeffrey F. DiBerto, Reid H.J. Olsen, and Bryan L. Roth

 

14. MERLIN: A BRET-Based Proximity Biosensor for Studying Mitochondria­–ER Contact Sites

            Hector Flores-Romero and Ana J. García-Sáez

 

15. Single Cell NanoBRET Imaging with Green-Range Halo-Tag Acceptor

            Ovia Thirukkumaran and Hideaki Mizuno

 

16. Method for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Indicators

            Mitsuru Hattori, Yukino Itoh, and Takeharu Nagai

 

17. BRET Sensors for Imaging Membrane Integrity of Microfluidically Generated Extracellular Vesicles

            Ramasamy Paulmurugan, Yi Liu, Uday Kumar Sukumar, Masamitsu Kanada, and Tarik F. Massoud

 

18. In Vivo Assessment of Protein-Protein Interactions Using BRET Assay

            Aaiyas Mujawar and Abhijit De

 

19. Live Cell Imaging of ATP Dynamics in Plant Cells

            Ryoichi Sato and Shinji Masuda

 

20. Bioluminescence Resonance Energy Transfer for Global DNA Methylation Quantification

            Natsumi Taka, Yuji Baba, Yuka Iwasaki, and Wataru Yoshida

 

21. In Vivo Bioluminescent Imaging of Bone Marrow Derived-Mesenchymal Stem Cells in Mice

            Prakash Gangadaran, Ji Min Oh, Ramya Lakshmi Rajendran, and Byeong-Cheol Ahn

 

22. In Vivo Imaging of Oxidative and Hypoxic Stresses in Mice Model of Amyotrophic Lateral Sclerosis

            Yasuyuki Ohta, Emi Nomura, Shinae Kizaka-Kondoh, and Koji Abe

 

Part IV: Instrumentation

 

23. ATP Sensing Paper with Smartphone Bioluminescence-Based Detection

            Maria Maddalena Calabretta, Ruslan Alvarez-Diduk, Elisa Michelini, and Arben Merkoçi

 

24. Organ Bioluminescence Imaging under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation

            Yuhei Higashi, Jun Homma, and Hidekazu Sekine

 

25. Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo

            Marina Sanaki-Matsumiya and Ryoichiro Kageyama

 

26. Bioluminescence-Optogenetics: A Practical Guide

            Matthew A. Stern, Henry Skelton, Alejandra M. Fernandez, Claire-Anne N. Gutekunst, Ken Berglund, and Robert E. Gross

 

27. Applications of Bioluminescence-Optogenetics in Rodent Models

            Matthew A. Stern, Henry Skelton, Alejandra M. Fernandez, Claire-Anne N. Gutekunst, Robert E. Gross, and Ken Berglund

 

28. One-Channel Microsliding Luminometer for Quantifying Low Energy Bioluminescent Lights

            Sung-Bae Kim and Ramasamy Paulmurugan

 

29. Compact Eight-Channel Light Sensing System for Bioassays

            Sung-Bae Kim, Sharon Seiko Hori, Negar Sadeghipour, Uday Kumar Sukumar, and Ramasamy Paulmurugan

 

30. Characterization of Firefly Flashes at Various Temperatures in Different Wavelength Regions

            Anurup Gohain Barua and Angana Goswami

 

31. Bioluminescent Monitoring of Circadian Rhythms in Isolated Mesophyll Cells of Arabidopsis at Single-Cell Level

            Shunji Nakamura and Tokitaka Oyama

 

Part V: Software

 

32. Exploring Phylogenetic Relationships and Divergence Times of Bioluminescent Species Using Genomic and Transcriptomic Data

            Danilo T. Amaral, Monique Romeiro-Brito, and Isabel A.S. Bonatelli