Bioluminescence: Methods and Protocols

ISBN-13: 9781603273206 / Angielski / Twarda / 2009 / 280 str.

Preston B. Rich

Opis

Part I I wasraised in a redbrick Baltimore row housewhere summer was marked by the ti- honored ritual of firefly-chasing - a backyard tradition that has endured the gene- tions.Amidtheexcitement, myfatheroftentoldthestoryofhow, whenhewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bugs as the dwindling light of dusk would permit. The very next morning, each 100-count glass jar of glowing crawling insects could be exchanged at the University for exactly one crisp dollar bill. Unrecognized at the time by my father, his joyous endeavors had contributed in a profoundwaytotheadvancedmolecularbiologicaltechniquesthatserveasthebasisfor thistextbook.In1947, WilliamMcElroyusedextractsfromthoseveryfirefliestodefine the fundamental reaction underlying the mystical phenomenon of luminescence, and published ''The energy source for bioluminescence in an isolated system'' in the Proceedings of the National Academy of Sciences. Inthedecadessincethatsummer, thestudyandapplicationofbioluminescencehave allowed us to leverage the enduring power of nature's elegance. We have painstakingly harnessedapowerfultoolthatenablesustoseekadeeperunderstandingofthecomplex mechanisms underpinning so many vital biologic systems. This second edition of Methods in Molecular Biology's Bioluminescence: Methods and Protocols serves as a re- ableandutilitariancompilationofthenewestandmostinnovativetechniquesthathave emerged in this rapidly expanding and progressively diverse field. We are indebted to the authors for their thoughtful contributions, inspired by their rigorous dedication to the science of bioluminescence, humbled by the unyielding supportofourcolleagues, andgratefulfortheopportunityprovidedusbyJohnandJan Walker.

Recenzje

From the reviews of the second edition: "This is a compilation of laboratory protocols dealing with diverse applications of bioluminescence in the biological sciences. ... The book is written primarily for scientists working in the field of biomedical sciences. ... It also provides a number of updated protocols and examples for practicing investigators interested in using bioluminescence in their research. ... This second edition should prove to be a valuable companion to all researchers interested in bioluminescence." (Ruben Mestril, Doody's Review Service, December, 2009)

Spis treści

1. Luminescent Probes and Visualization of Bioluminescence Elisa Michelini, Luca Cevenini, Laura Mezzanotte, and Aldo Roda 2. Validation of Bioluminescent Imaging Techniques John Virostko and E. Duco Jansen 3. Assessment of Extracellular ATP Concentrations Lucia Seminario-Vidal, Eduardo R. Lazarowski, and Seiko F. Okada 4. High Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden Angelina Contero, Edmond Richer, Ana Gondim, and Ralph P. Mason 5. Fluorescence Imaging of Tumors with 'Smart' pH-Activatable Targeted Probes Daisuke Asanuma, Hisataka Kobayashi, Tetsuo Nagano, and Yasuteru Urano 6. Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, B. Christoffer Lagerholm, Alan S. Waggoner, and Marcel P. Bruchez 7. Bioluminescent Imaging of Transplanted Islets Xiaojuan Chen and Dixon B. Kaufman 8. Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cell Survival, Proliferation, and Fate Kitchener D. Wilson, Mei Huang, and Joseph C. Wu 9. Detection of Apoptosis Using Cyclic Luciferase in Living Mammals Akira Kanno, Yoshio Umezawa, and Takeaki Ozawa 10. Non-Invasive Bioluminescent Imaging of Infections Javier S. Burgos 11. Real-Time Bioluminescence Imaging of Viral Pathogenesis Kathryn E. Luker and Gary D. Luker 12. Bioluminescent Monitoring of in vivo Colonization and Clearance Dynamics by Light-Emitting Bacteria Siouxsie Wiles, Brian D. Robertson, Gad Frankel, and Angela Kerton 13. Quantitative In Vivo Imaging of Non-Viral Mediated Gene Expression and RNAi Mediated Knockdown Garrett R. Rettig and Kevin G. Rice 14. Analysis of Protein-Protein Interactions Using Bioluminescence Resonance Energy Transfer Kevin D.G. Pfleger 15. Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay Akira Kanno, Takeaki Ozawa, and Yoshio Umezawa 16. Bioluminescence Analysis of Smad-Dependent TGF-b Signaling in Live Mice Jian Luo and Tony Wyss-Coray 17. Bioluminescence Imaging of Calcium Oscillations Inside Intracellular Organelles Carlos Villalobos, María Teresa Alonso, and Javier García-Sancho 18. Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) Assays Mélanie Robitaille, Isabelle Héroux, Alessandra Baragli, and Terence E. Hébert 19. PIN-G Reporter for Imaging and Defining Trafficking Signals in Membrane Proteins Lynn Mckeown, Vicky C. Jones, and Owen T. Jones 20. Imaging ß-Galactosidase Activity in vivo Using Sequential Reporter-Enzyme Luminescence Georges von Degenfeld, Tom S. Wehrman, and Helen M. Blau

Blurb

Through the study and application of bioluminescence, scientists have painstakingly harnessed a powerful tool that enables us to seek a deeper understanding of the complex mechanisms underpinning so many vital biologic systems. In this fully revised and updated second edition of Bioluminescence: Methods and Protocols, expert researchers contribute a readable and utilitarian compilation of the newest and most innovative techniques that have emerged in this rapidly expanding and progressively diverse field including methods to assess cell trafficking, protein-protein interactions, intracellular signaling, and apoptosis. Also opening up the possibility to visualize and quantify biological mechanisms in real-time and in in vivo settings, the volume also describes the in vivo study of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials, equipment, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescence: Methods and Protocols, Second Edition provides protocols that are detailed enough to be followed and adapted by scientific teams who have no previous expertise in bioluminescence in order to foster the potentially numerous breakthroughs and new applications from basic to applied science and medicine that must continue to be developed.