Bioluminescence: Methods and Protocols

ISBN-13: 9781493938117 / Angielski / Twarda / 2016 / 314 str.

Sung-Bae Kim

Opis

This volume presents detailed laboratory protocols regarding the three major route technologies luciferases, efficient optical probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a useful complement to the first and second edition for new and experienced researchers alike.

Spis treści

 Label-free Cell Phenotypic Identification of D-Luciferin as an Agonist for GPR35.- Synthetic Bioluminescent Coelenterazine Derivatives.- Molecular Cloning of Secreted Luciferases from Marine Planktonic Copepods.-  How to Fabricate Functional Artificial Luciferases for Bioassays.- Quantum Yield Determination Based on Photon Number Measurement, Protocols for Firefly Bioluminescence Reactions.- Bioluminescent Ligand-Receptor Binding Assays for Protein or Peptide Hormones.- Bioluminogenic Imaging of Aminopeptidase N in vitro and in vivo.-  Firefly Luciferase-based Sequential Bioluminescence Resonance Energy Transfer (BRET)-Fluorescence Resonance Energy Transfer (FRET) Protease Assays.- Monitoring Intracellular pH Change with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and Mammalian Cells.- A Protein–Protein Interaction Assay FlimPIA Based on the Functional Complementation of Mutant Firefly Luciferases.- Single-chain Probes for Illuminating Androgenicity of Chemicals.- Multicolor Imaging of Bifacial Activities of Estrogens.-  Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor.-  Fabrication of Molecular Strain Probes for Illuminating Protein–Protein Interactions.- An ALuc-based Molecular Tension Probe for Sensing Intramolecular Protein–Protein Interactions.- Live Cell Bioluminescence Imaging in Temporal Reaction of G Protein-coupled Receptor for High-throughput Screening and Analysis.-  Imaging Histone Methylations in Living Animals.-  Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays.- In Vivo Bioluminescent Imaging of ATP-Binding Cassette Transporter-Mediated Efflux at the Blood-Brain Barrier.- Theranostic Imaging of Cancer Gene Therapy.-  Development of a Multicolor Bioluminescence Imaging Platform to Simultaneously Investigate Transcription Factor NF-κB Signaling and Apoptosis .- A Multichannel Bioluminescence Determination platform for Bioassays.- A Bioluminescence Assay System for Imaging Metal Cationic Activities in Urban Aerosols.- Luminescence Imaging: (a) Multi-color Visualization of Ca²⁺ Dynamics in Different Cellular Compartments and (b) Video-rate Tumour Detection in a Freely Moving Mouse.-  Photon Counting System for High-sensitivity Detection of Bioluminescence at Optical Fiber End.