Bioconjugation Protocols: Strategies and Methods

ISBN-13: 9781617791505 / Angielski / Twarda / 2011 / 605 str.

Sonny S. Mark

Opis

Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of "Bioconjugation Protocols: Strategies and Methods," expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful "Methods in Molecular Biology"TM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and highly practical, "Bioconjugation Protocols: Strategies and Methods, Second Edition" offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.

Spis treści

Part I: Protein Conjugates

 

1. Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities

            Edward A. Lemke

 

2. Enzymatically-Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase

            Ahmed Besheer, Thomas C. Hertel, Jörg Kressler, Karsten Mäder, and Markus Pietzsch

 

3. Synthesis of Drug/Dye-Incorporated Polymer-Protein Hybrids

            Sukanta Dolai, Wei Shi, Bikash Mondal, and Krishnaswami Raja

 

4. Dye/DNA Conjugates as Multiple Labels for Antibodies in Sensitive Fluorescence Immunoassays

            Qin Zhang, Shengchao Zhu, and Liang-Hong Guo

 

5. Chemoselective Modification of Viral Proteins Bearing Metabolically Introduced ‘Clickable’ Amino Acids and Sugars

            Partha S. Banerjee and Isaac S. Carrico

 

6. Preparation of Peptide and Other Biomolecular Conjugates through Chemoselective Ligations

            Mathieu Galibert, Olivier Renaudet, Didier Boturyn, and Pascal Dumy

 

7. New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed Covalent Protein Labeling

            Noriho Kamiya and Hiroki Abe

 

8. Covalent Conjugation of Poly(Ethylene Glycol) to Proteins and Peptides: Strategies And Methods

            Anna Mero, Chiara Clementi, Francesco M. Veronese, and Gianfranco Pasut

 

9. Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag to Proteins Using Protein Trans-Splicing

            Tulika Dhar, Thomas Kurpiers, and Henning D. Mootz

 

Part II: Nucleic Acid Conjugates

 

10. Polyethylenimine Bioconjugates for Imaging and DNA Delivery In Vivo

            Andrea Masotti and Francesco Pampaloni

 

11. Synthesis of a Glycomimetic Oligonucleotide Conjugate By 1,3-Dipolar Cycloaddition

            Gwladys Pourceau, Albert Meyer, Jean-Jacques Vasseur, and François Morvan

 

12. Site-Specific DNA Labeling by Staudinger Ligation

            Samuel H. Weisbrod, Anna Baccaro, and Andreas Marx

 

13. Improved Cellular Uptake of Antisense Peptide Nucleic Acids (PNAs) by Conjugation to a Cell-Penetrating Peptide and a Lipid Domain

            Takehiko Shiraishi and Peter E. Nielsen

 

14. Synthesis of Oligonucleotide-Peptide Conjugates for Biomedical and Technological Applications

            Anna Aviñó, Santiago Grijalvo, Sónia Pérez-Rentero, Alejandra Garibotti, Montserrat Terrazas, and Ramon Eritja

 

15. Amphiphilic DNA Block Copolymers: Nucleic Acid-Polymer Hybrid Materials for Diagnostics and Biomedicine

            Jan Zimmermann, Minseok Kwak, Andrew J. Musser, and Andreas Herrmann

 

Part III: Glycosyl and Lipid Conjugates

 

16. Chemically Selective Liposome Surface Glyco-Functionalization

            Hailong Zhang, Yong Ma, and Xue-Long Sun

 

17. Bioconjugation Using Mutant Glycosyltransferases for the Site-Specific Labeling of Biomolecules with Sugars Carrying Chemical Handles

            Boopathy Ramakrishnan, Elizabeth Boeggeman, Marta Pasek, and Pradman K. Qasba

 

18. Lipid-Core-Peptide System for Self-Adjuvanting Synthetic Vaccine Delivery

            Mariusz Skwarczynski and Istvan Toth

 

19. Coupling Carbohydrates to Proteins for Glycoconjugate Vaccine Development Using a Pentenoyl Group as a Convenient Linker

            Qianli Wang and Zhongwu Guo

 

20. Conjugation of LPS-Derived Oligosaccharides to Proteins Using Oxime Chemistry

            Joanna Kubler-Kielb

 

21. Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast

            Junpeng Xiao and Thomas J. Tolbert

 

22. On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-linked Oligosaccharide

            Rui Chen and Thomas J. Tolbert

 

23. Design and Synthesis of Novel Functional Lipid-Based Bioconjugates for Drug Delivery and Other Applications

            Rupa R. Sawant and Vladimir P. Torchilin

 

Part IV: Biofunctionalization of Surfaces and Thin Films

 

24. Chemical Functionalization and Bioconjugation Strategies for Atomic Force Microscope (AFM) Cantilevers

            Magnus Bergkvist and Nathaniel C. Cady

 

25. Chemoselective Protein and Peptide Immobilization on Biosensor Surfaces

            Edith H.M. Lempens, Brett A. Helms, and Maarten Merkx

 

26. Fabrication of Dynamic Self-Assembled Monolayers for Cell Migration and Adhesion Studies

            Nathan P. Westcott and Muhammad N. Yousaf

 

27. DNA Detection Using Functionalized Conducting Polymers

            Jadranka Travas-Sejdic, Hui Peng, Hsiao-hua Yu, and Shyh-Chyang Luo

 

28. Preparation and Dynamic Patterning Of Supported Lipid Membranes Mimicking Cell Membranes

            Stefan Kaufmann, Karthik Kumar, and Erik Reimhult

 

29. Enzyme Immobilization on Reactive Polymer Films

            Ana L. Cordeiro, Tilo Pompe, Mariana Tasso, Katrin Salchert, and Carsten Werner

 

30. Characterization of Protein–Membrane Binding Interactions Via a Microplate Assay Employing Whole Liposome Immobilization

            Matthew D. Smith and Michael D. Best

 

31. A Bioconjugated Phospholipid Polymer Biointerface with Nanometer-Scaled Structure for Highly Sensitive Immunoassays

            Kazuki Nishizawa, Madoka Takai, and Kazuhiko Ishihara

 

Part V: Biofunctionalization of Nanostructures

 

32. Purification, Functionalization, and Bioconjugation of Carbon Nanotubes

            John H.T. Luong, Keith B. Male, Khaled A. Mahmoud, and Fwu-Shan Sheu

 

33. Functional Integration of Membrane Proteins with Nanotube and Nanowire Transistor Devices

            Aleksandr Noy, Alexander B. Artyukhin, Shih-Chieh Huang, Julio A. Martinez, and Nipun Misra

 

34. Single-Step Conjugation of Antibodies to Quantum Dots for Labeling Cell Surface Receptors in Mammalian Cells

            Gopal Iyer, Jianmin Xu, and Shimon Weiss

 

35. A Practical Strategy for Constructing Nanodrugs Using Carbon Nanotubes as Carriers

            Wei Wu and Xiqun Jiang

 

36. Design and Synthesis of Biofunctionalized Metallic/Magnetic Nanomaterials

            Eun-Kyung Lim, Seungjoo Haam, Kwangyeol Lee, and Yong-Min Huh