12. Hydrogel Beads of Natural Polymers as a Potential Vehicle for Colon-Targeted Drug Delivery
Janarthanan Pushpamalar, Thenapakiam Sathasivam, and Michelle Claire Gugler
13. Scaffold-Based Delivery of CRISPR/Cas9 Ribonucleoproteins for Genome Editing
Wai Hon Chooi, Jiah Shin Chin, and Sing Yian Chew
14. Correction of Hemoglobin E/Beta-Thalassemia Patient-Derived iPSCs Using CRISPR/Cas9
Methichit Wattanapanitch
15. CRISPR/Cas9-Mediated GFP Reporter Knock-In in K562 and Raji Cell Lines for Tracking Immune Cell Killing Assay
Nontaphat Thongsin and Methichit Wattanapanitch
Part V: Analyzing and Profiling Gene Expression
16. Shotgun Proteomics and Mass Spectrometry as a Tool for Protein Identification and Profiling of Bio-Carrier-Based Therapeutics on Human Cancer Cells
Syafiq Asnawi Zainal Abidin, Iekhsan Othman, and Rakesh Naidu
This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the traditional viral and non-viral systems and explores ideas for blending the best features of bacteria, nanoparticles, peptides, and hybrid systems for the delivery of biomaterials into cells, as well as a chapter on the application of shotgun proteomics and mass spectrometry as a tool to analyze the proteomic profile changes in cells that result from these interventions. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Bio-Carrier Vectors: Methods and Protocols serves as an ideal guide for researchers working toward harnessing the power of multiple vectors in gene and drug delivery.