Bio-Carrier Vectors: Methods and Protocols

ISBN-13: 9781071609422 / Angielski / Twarda / 2020 / 243 str.

Kumaran Narayanan

Spis treści

Part I: Bacterial Vectors

 

1. Improving E. coli Bactofection by Expression of Bacteriophage ΦX174 Gene E

            Dongwon Park, Andrew Hill, and Blaine A. Pfeifer

 

2. Improved DNA Delivery Efficiency of Bacterial Vectors by Co-Delivery with Exogenous Lipid and Antimicrobial Reagents

            Andrew N. Osahor and Kumaran Narayanan

 

3. Visualization of Bacteria-Mediated Gene Delivery Using High Resolution Electron and Confocal Microscopy

            Andrew N. Osahor, Allan Wee Ren Ng, and Kumaran Narayanan

 

Part II: Nanoparticles

 

4. Assessing Nucleic Acid:Cationic Nanoparticle Interaction for Gene Delivery

            Moganavelli Singh

 

5. Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability and Efficacy of Chemotherapeutic Drugs

            Xuemei Xie, Dan He, Yan Wu, Tingting Wang, Cailing Zhong, and Jingqing Zhang

 

6. Magnetic Nanoparticles as a Carrier of dsRNA for Gene Therapy

            Małgorzata Grabowska, Bartosz F. Grześkowiak, Katarzyna Rolle, and Radosław Mrówczyński

 

Part III: Peptide Vehicles

 

7. Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells through Protein Transduction of Transcription Factors

            Taku Kaitsuka and Kazuhito Tomizawa

 

8. Cardiac Targeting Peptide: From Identification to Validation to Mechanism of Transduction

            Kyle S. Feldman, Maria P. Pavlou, and Maliha Zahid

 

9. Lactoferricin-Derived L5a Cell-Penetrating Peptide for Delivery of DNA into Cells

            Natalie J. Holl, Moumita Dey, Yue-Wern Huang, Shiow-Her Chiou, and Han-Jung Lee

 

10. Delivery of Peptide Nucleic Acids Using an Argininocalix[4]arene as Vector

            Alessia Finotti, Jessica Gasparello, Alessandro Casnati, Roberto Corradini, Roberto Gambari, and Francesco Sansone

 

Part IV: Hybrid Carriers and Other Bio Agents

 

11. Cell-Derived Nanovesicles as Exosome-Mimetics for Drug Delivery Purposes: Uses and Recommendations

            Yi-Hsuan Ou, Shui Zou, Wei Jiang Goh, Jiong-Wei Wang, Matthias Wacker, Bertrand Czarny, and Giorgia Pastorin

 

12. Hydrogel Beads of Natural Polymers as a Potential Vehicle for Colon-Targeted Drug Delivery

            Janarthanan Pushpamalar, Thenapakiam Sathasivam, and Michelle Claire Gugler

 

13. Scaffold-Based Delivery of CRISPR/Cas9 Ribonucleoproteins for Genome Editing

            Wai Hon Chooi, Jiah Shin Chin, and Sing Yian Chew

 

14. Correction of Hemoglobin E/Beta-Thalassemia Patient-Derived iPSCs Using CRISPR/Cas9

            Methichit Wattanapanitch

 

15. CRISPR/Cas9-Mediated GFP Reporter Knock-In in K562 and Raji Cell Lines for Tracking Immune Cell Killing Assay

            Nontaphat Thongsin and Methichit Wattanapanitch

 

Part V: Analyzing and Profiling Gene Expression

 

16. Shotgun Proteomics and Mass Spectrometry as a Tool for Protein Identification and Profiling of Bio-Carrier-Based Therapeutics on Human Cancer Cells

            Syafiq Asnawi Zainal Abidin, Iekhsan Othman, and Rakesh Naidu

Blurb

This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the traditional viral and non-viral systems and explores ideas for blending the best features of bacteria, nanoparticles, peptides, and hybrid systems for the delivery of biomaterials into cells, as well as a chapter on the application of shotgun proteomics and mass spectrometry as a tool to analyze the proteomic profile changes in cells that result from these interventions. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Bio-Carrier Vectors: Methods and Protocols serves as an ideal guide for researchers working toward harnessing the power of multiple vectors in gene and drug delivery.