Basic Life Science Methods: A Laboratory Manual for Students and Researchers

ISBN-13: 9780443191749 / Angielski / Miękka / 2022 / 230 str.

Javeed Ahmad Tantray; Sheikh Mansoor; Rasy Fayaz Choh Wani

Spis treści

1. Calculations of Molarity and Normality 2. Glassware washing and sterilization techniques 3. pH Meter: Its use and calibration 4. Principle and working of Autoclave 5. Principle and working of Centrifuge 6. Principles and working of Laminar 7. Estimation of Total cholesterol (Flegg 1972) 8. Estimation of Creatinine 9. Estimation of blood Urea 10. Estimation of blood glucose By GOD - POD method 11. Determination of Albumin, and Globulin in Plasma 12. Quantitative determination of Homocysteine 13. Estimation of serum bilirubin 14. Estimation of diagnostic enzyme ALT 15. Estimation of diagnostic enzyme AST 16. To Estimate Protein by Lowry's Method 17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method 18. To perform quantitative determination of total protein in Serum/plasma 19. Enzyme Activity Using Starch assay 20. To Estimate Protein by Bradford's Assay 21. To Identify a Protein Sample Using SDS-PAGE 22. Genomic DNA isolation from whole blood 23. PCR for gene amplification 24. Agarose Gel Electrophoresis (AGE) 25. RNA Isolation from Human tissue 26. Real-time polymerase chain reaction (RT-qPCR) 27. Preparation of competent cells by CaCl2 treatment 28. RNA Isolation from Plant tissues 29. DNA isolation from the bacterial culture broth 30. DNA isolation from fungal Mycelium 31. Quantification of the DNA using spectrophotometer 32. Formaldehyde gel electrophoresis of the isolated RNA sample 33. Paper chromatography of different amino acid 34. Separation of plants pigments using Column Chromatography 35. Separation of plant pigment by Thin Layer Chromatography 36. Chromatin Immunoprecipitation (ChIP) Protocol 37. Types of Media 38. Preparation of Nutrient Agar Media 39. Serial Dilution of the Bacterial Cells 40. Spread Plate Method of The Bacterial Cells 41. Streak Plate Method 42. Identified Bacterial Colony in Culture Broth 43. Pour plate method for bacterial colony counting 44. Gram Staining of Bacteria 45. Casein hydrolysis test 46. Antibiotic resistance test by Agar Well Diffusion Method

O autorze

Dr. Javeed Ahmad Tantray's current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist awards both at national and international level. He received the Best Abstract Award at the 4th Asia Pacific CardioMetabolic Syndrome Congress held by the Korean Society of CardioMetabolic Syndrome,with an award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on "Assembly and usage of Foldscope”. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K). Nighat Un Nissa has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir. Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms. Rasy is currently pursuing Ph.D. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on research topic "Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”. Dr. Sheikh Mansoor Shafi is a postdoctoral researcher, presently working at the SK- Institute of Medical Sciences Soura Srinagar, India. Previously he was working as project associate-I at the CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). He obtained his PhD from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu. Dr. Mansoor has made important contributions to his field and has an impressive lab experience in biochemistry, molecular biology, and genetics. He has published more than 30 research and review papers in reputed journals. He has published more than 10 book chapters, 3 books and he has filed one patent application.