Basic Concepts in Clinical Biochemistry: A Practical Guide

ISBN-13: 9789811340864 / Angielski / Miękka / 2019 / 175 str.

Vijay Kumar; Kiran Dip Gill

Spis treści

1.​ Common clinical laboratory hazards and waste disposal

2.Blood collection and preservation 

3. Quality Control in laboratory

4. Automation in clinical laboratory

5. Photometry-Colorimeter and Spectrophotometer

6. Preparation of general laboratory solutions and buffers

7. Examination of urine for normal constituents

8. To perform qualitative tests for urinary proteins.

9. To determine the quantity of proteins in urine sample using Biuret reaction

10. To estimate the amount of total protein and albumin in serum and to find A/G ratio       

11. To perform qualitative test for reducing substances in urine

12. Quantitative analysis of reducing sugars in urine

13. Estimation of blood glucose levels by Glucose Oxidase method

14. Estimation of blood glucose levels by Folin and Wu method

15. To perform Glucose Tolerance Test

16. Estimation of urea in serum and urine

17. To determine urea clearance

18. To estimate creatinine level in serum and urine by Jaffe’s reaction  

19. To determine creatinine clearance

20. To determine the uric acid concentration in serum and urine

21. Estimation of total calcium in serum and urine

22. Estimation of inorganic phosphorus in serum and urine

23. To estimate the amount of total cholesterol in serum.

24. To estimate total and direct bilirubin in serum

25. To determine Alanine and  Aspartate Transaminases activity in serum     

26. To estimate the activity of alkaline phosphatase in serum

27. To estimate the activity of acid phosphatase in serum

28. To determine serum and urinary amylase activity.

29. To estimate the activity of lipase in serum.

30. Qualitative analysis of ketone bodies in urine.

31. Qualitative test for bile pigments and urobilinogen in urine.

32. Determination of total lactate dehydrogenase activity in serum

33. To measure the activity of Creatine kinase-MB and Total Creatine kinase in serum.

34. Analysis of Cerebrospinal Fluid for proteins and sugars

35. To analyze lipid profile from given serum sample

36. To determine sodium and potassium in serum by using Flame photometry

37. To perform Radioimmunoassay

38. To perform Enzyme linked Immunosorbant assay

39. Some important case studies

O autorze

Prof. Kiran Dip Gill obtained her Ph.D. degree in Biochemistry from Punjab Agriculture University, Ludhiana, India in 1974. She was a postdoctoral fellow at University of Gottingen, Germany in 1977-1978.  In 1981, she joined department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India. She has taught Biochemistry to post-graduate students for almost 35 years. She was also in-charge of clinical biochemistry lab, where biochemical investigations are done routinely. She has authored four chapters in books and published more than 125 research papers in her career. She is recipient of several awards for her contribution in research work.

Dr. Vijay Kumar obtained his Ph.D. degree in Biochemistry under the guidance of Prof. Kiran Dip Gill from Post Graduate Institute of Medical Education and Research, Chandigarh in 2009. In 2010, he joined Maharshi Dayanand University, Rohtak. Since then, he is actively involved in teaching Biochemistry to post-graduate students. He has published more than 20 research papers and three review articles in reputed journals. 

Blurb

This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.