Bacterial Cell Surfaces: Methods and Protocols

ISBN-13: 9781493959938 / Angielski / Miękka / 2016 / 399 str.

Anne H. Delcour

Opis

This Methods in Molecular Biology (TM) book gathers tools for studying protein structure and function and enzymatic activities, purifying and analyzing of macromolecules and their complexes and investigating regulatory mechanisms and cell biological processes.

Spis treści

1. A Bird’s Eye View of the Bacterial Landscape

            Natividad Ruiz

 

2. Visualizing the Bacterial Cell Surface: An Overview

            Harald Engelhardt

 

3. Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems

            Nadine S. Henderson and David G. Thanassi

 

4. Experimental Manipulation of the Microbial Functional Amyloid Called Curli

            Yizhou Zhou, Daniel R. Smith, David A. Hufnagel, and Matthew R. Chapman

 

5. Visualization of Gram-Positive Bacterial Pili

            Chungyu Chang, I-Hsiu Huang, Antoni P. Hendrickx, and Hung Ton-That

 

6. Single Cell Microfluidic Studies of Bacterial Motility

            Adrien Ducret, Olivier Théodoly, and Tâm Mignot

 

7. Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule

            Susan M. Steenbergen and Eric R. Vimr

 

8. Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy

            Wei Hu, Renate Lux, and Wenyuan Shi

 

9. Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance

            Elena B. Tikhonova and Helen I. Zgurskaya

 

10. Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System

            Tanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, and Maria Sandkvist

 

11. Pore Formation by T3SS Translocators: Liposome Leakage Assay

            Eric Faudry, Caroline Perdu, and Ina Attrée

 

12. Isolation of Bacterial Type IV Machine Subassemblies

            Mayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, and Peter J. Christie

 

13. Production and Crystallization of Bacterial Type V Secretion Proteins

            Hye-Jeong Yeo

 

14. Assembly of Bacterial Outer Membrane Proteins

            Jan Grijpstra, Martine P. Bos, and Jan Tommassen

 

15. The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization

            Jessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, and M. Stephen Trent

 

16. Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles

            Halima Chutkan, Ian MacDonald, Andrew Manning, and Meta J. Kuehn

 

17. In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate

            Jacob Biboy, Nhat Khai Bui, and Waldemar Vollmer

 

18. Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes

            Filipe Carvalho, María Graciela Pucciarelli, Francisco García-del Portillo, Didier Cabanes, and Pascale Cossart

 

19. Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis

            Anna E. Grzegorzewicz and Mary Jackson

 

20. Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues

            Katleen Denoncin, Valerie Nicolaes, Seung-Hyun Cho, Pauline Leverrier, and Jean-Francois Collet

 

21. Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress Response

            Julia L. Wong, Stefanie L. Vogt, and Tracy L. Raivio

 

22. Isolation of Bacteria Envelope Proteins

            Shu Quan, Annie Hiniker, Jean-Francois Collet, and James C.A. Bardwell

 

23. Patch Clamp Electrophysiology for the Study of Bacterial Ion Channels in Giant Spheroplasts of E. coli

            Boris Martinac, Paul R. Rohde, Charles G. Cranfield, and Takeshi Nomura

 

24. Electrophysiological Characterization of Bacterial Pore-Forming Proteins in Planar Lipid Bilayers

            Owen S. Mapingire, Beau Wager, and Anne H. Delcour

Blurb

In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.