Atomic Force Microscopy in Biomedical Research: Methods and Protocols

ISBN-13: 9781617791048 / Angielski / Twarda / 2011 / 524 str.

Pier Carlo Braga; Davide Ricci

Opis

With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.

Spis treści

Part I: The Basics of Atomic Force Microscopy

 

1. How the Atomic Force Microscope Works

            Bruno Torre, Davide Ricci, and Pier Carlo Braga

 

2. Measurement Methods in Atomic Force Microscopy

            Bruno Torre, Claudio Canale, Davide Ricci, and Pier Carlo Braga

 

3. Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging

            Claudio Canale, Bruno Torre, Davide Ricci, and Pier Carlo Braga

 

Part II: Molecule Imaging

 

4. Imaging the Spatial Orientation of Subunits within Membrane Receptors by Atomic Force Microscopy

            Stewart M Carnally, J. Michael Edwardson, and Nelson P. Barrera

 

5. High Resolution Imaging of Immunoglobulin G (IgG) Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air

            Sergio Santos and Neil H. Thomson

 

6. Atomic Force Microscopy of Ex vivo Amyloid Fibrils

            Claudio Canale, Annalisa Relini, and Alessandra Gliozzi

 

7. Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy

            Clemens M. Franz and Daniel J. Muller

 

8. Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid

            Osamu Hoshi and Tatsuo Ushiki

 

9. Atomic Force Microscopy of Proteasome Assemblies

            Maria Gaczynska and Pawel A. Osmulski

 

10. Atomic Force Microscopy of Isolated Mitochondria

            Bradley E. Layton and M. Brent Boyd

 

11. Imaging Native Membrane Proteins Using the Atomic Force Microscope

            Andreas Engel

 

Part III: Nanoscale Surface Analysis and Cell Imaging

 

12. Atomic Force Microscopy Investigation of Viruses

            Alexander McPherson and Yurii G. Kuznetsov

 

13. Determination of the Kinetic On and -Off Rate of Single Virus-Cell Interactions

            Christian Rankl, Linda Wildling, Isabel Neundlinger, Ferry Kienberger, Hermann Gruber, Dieter Blaas, and Peter Hinterdorfer

 

14. Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites

            Wanderley de Souza, Gustavo M. Rocha, Kildare Miranda, Paulo M. Bisch, and Gilberto Weissmuller

 

15. Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy

            Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer

 

16. The Growth Cones of Living Neurons Probed by the Atomic Force Microscope

            Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco

 

17. Highlights on Ultrastructural Pathology of Human Sperm

            Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna

 

18. High-speed Atomic Force Microscopy and Biomolecular Processes

            Takayuki Uchihashi and Toshio Ando

 

Part IV: Non-Topographical Applications (Force-Spectroscopy)

 

19. Atomic Force Microscopy in Mechanobiology: Measuring Micro-Elastic Heterogeneity of Living Cells

            Evren U. Azeloglu and Kevin D. Costa

 

20. Force-Clamp Measurements of Receptor-Ligand Interactions

            Félix Rico, Calvin Chu, and Vincent T. Moy

 

21. Measuring Cell Adhesion Forces: Theory and Principles

            Martin Benoit and Christine Selhuber-Unkel

 

22. Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces

            Ting Cao, Haiying Tang, Xuemei Liang, Anfeng Wang, Gregory W. Auner, Steven O. Salley, and K.Y. Simon Ng

 

Part V: Investigating Drug Action

 

23. Imaging Bacterial Shape, Surface, and Appendages Before and After Treatment with Antibiotics

            Pier Carlo Braga and Davide Ricci

 

24. Thymol-Induced Alterations in Candida albicans Imaged by Atomic Force Microscopy

            Pier Carlo Braga and Davide Ricci

 

25. Atomic Force Microscope-Enabled Studies of Integrin-Extracellular Matrix Interactions in Vascular Smooth Muscle and Endothelial Cells

            Zhe Sun and Gerald A. Meininger

 

26. Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin

            Zhongdang Xiao, Lili Cao, Dan Zhu, and Zuhong Lu

 

Part VI: Atomic Force Microscopy as a Nanotool

 

27. Combined Atomic Force Microscopy and Fluorescence Microscopy

            Miklós S.Z. Kellermayer

 

28. Chemical Modifications of Atomic Force Microscopy Tips

            Régis Barattin and Normand Voyer

 

29. Atomic Force Microscopy as Nanorobot

            Ning Xi, Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Donna H. Wang, Kristina Seiffert-Sinha, Animesh A. Sinha, Guangyong Li, and Lianqing Liu