Assessment of Genetic Diversity among local Xanthomonas isolates

ISBN-13: 9783846535301 / Angielski / Miękka / 76 str.

Sabin Fatima; Rukhsana Bajwa

Opis

The genus Xanthomonas is significant due to its phytopathogenic diversity and contrasting phenotypic uniformity. Appropriate information on classification and nomenclature of genus Xanthomonas is essential to ensure effective disease management. In this research work isolates were distinguished through enzyme production potential and by using PCR techniques i.e. RAPD and ISSR. Genetic profile of pathogenic and non pathogenic isolates was checked in order to have a complete picture attributed generally to geographical distribution and or pathogenecity of various Xanthomonas species/ pathovars. Authenticated information on molecular and biochemical diversity among species and pathovars will be advantageous for Plant breeding programs. Cluster dendrogram obtained from combined data of RAPD and ISSR showed that genetic diversity exists in local isolates of Xanthomonas. In terms of percentage similarity values the genomic variation was found to be in the range of 29.29% - 100% among the isolates. This work will support the knowledge on characterization of some indigenous Xanthomonas species and inter/ intra specific variations among them.

O autorze

Sabin Fatima, Ph.D Scholar at Institute of Agricultural Sciences, University of the Punjab, Pakistan. Professional skills include DNA isolation, biochemical assays, genetic variation analysis, 16S rRNA gene targeting and SDS- PAGE analysis. Prof. Dr. Rukhsana Bajwa, Ex- Director, Institute of Agricultural Sciences has over 200 publications.