Array Comparative Genomic Hybridization: Protocols and Applications

ISBN-13: 9781493959556 / Angielski / Miękka / 2016 / 382 str.

Diponkar Banerjee; Sohrab P. Shah

Opis

This Methods in Molecular Biology (TM) book explores the scope of what is possible today in documenting abnormalities associated with several human cancers. Offers materials and reagents lists, step-by-step protocols, troubleshooting tips, pitfalls and more.

Spis treści

1. Array Comparative Genomic Hybridization: An Overview of Protocols, Applications and Technology Trends

            Diponkar Banerjee

 

2. Ultra Dense Array CGH and Discovery of Micro-Copy Number Alterations and Gene Fusions in the Cancer Genome

            Ewa Przybytkowski, Adriana Aguilar-Mahecha, Sheida Nabavi, Peter J. Tonellato, and Mark Basik

 

3. Epigenomics: Sequencing the Methylome

            Martin Hirst

 

4. Application of Array Comparative Genomic Hybridization in Chronic Myeloid Leukemia

            Seonyang Park, Youngil Koh, Seung-Hyun Jung, and Yeun-Jun Chung

 

5. The Use of Cytogenetic Microarrays in Myelodysplastic Syndrome Characterization

            Lisa G. Shaffer, Blake C. Ballif, and Roger A. Schultz

 

6. CGH Protocols - Chronic Lymphocytic Leukemia

            Esteban Braggio, Rafael Fonseca, and Neil E. Kay

 

7. Analysis of Acquire Genomic Copy Number Aberrations and Regions of Loss of Heterozygosity in Acute Myelogenous Leukemia Fenomes using Affymetrix SNP 6.0 Arrays and Supporting Software Tools

            Peter D. Ouillete, Kerby A. Shedden, Cheng Li, and Sami N. Malek

 

8. Clinical Applications of BAC Array-CGH to the Study of Diffuse Large B-Cell Lymphomas

            Cristina Robledo, Juan Luis García, and Jesús M. Hernández

 

9. Genomic Profiling of Mantle Cell Lymphoma

            Melissa Rieger and Jose A. Martinez-Climent

 

10. Copy Number Analysis in EBV-Positive Nodal Peripheral T-Cell Lymphoma, Unspecified

            Kyusam Choi, Hyunjeong Ju, and Young Hyeh Ko

 

11. Mycosis Fungoides and Sézary Syndrome

            Blanca Espinet and Rocío Salgado

 

12. Array CGH Reveals Clonal Evolution of Adult T Cell Leukemia/Lymphoma

            Akira Umino and Masao Seto

 

13. Array-CGH Analysis of Cutaneous Anaplastic Large Cell Lymphoma

            K. Szuhai, R. Van Doorn, and C.P. Tensen

 

14. Genome Wide DNA-Profiling of HIV-Related B-Cell Lymphomas

            Andrea Rinaldi, Daniela Capello, Emanuele Zucca, Gianluca Gaidano, and Franceso Bertoni

 

15. Array Comparative Genomic Hybridization in Osteosarcoma

            Bekim Sadijovi, Paul C. Park, Shamini Selvarajah, and Maria Zielenska

 

16. Comparative Genomic Hybridization of Wilms' Tumor

            Shahrad Rod Rassekh and Evica Rajcan-Separovic

 

17. Array-CGH in Childhood MDS

            Marcel Tauscher, Inka Praulich, and Doris Steinemann

 

18. Non-Familial Breast Cancer Subtypes

            Markus Ringnér, Johan Staaf, and Göran Jönsson

 

19. Applications of Array-CGH for Lung Cancer

            Kenneth J. Craddock, Wan L. Lam, and Ming-Sound Tsao

 

20. Array CGH in Brain Tumours

            Gayatry Mohapatra, Julia Sharma, and Stephen Yip

 

21. Finding Common Regions of Alteration in Copy Number Data

            Oscar M. Rueda, Ramon Diaz-Uriarte, and Carlos Caldas

 

22. Distinguishing Somatic and Germline Copy Number Events in Cancer Patient DNA Hybridized to Whole Genome SNP Genotyping Arrays

            Gavin Ha and Sohrab Shah

Blurb

The earliest descriptions of human chromosomes initiated the genomics revolution that is now upon us. Array Comparative Genomic Hybridization: Protocols and Applications explores the scope of what is now possible as far as documenting abnormalities associated with several human cancers. While the technology for interrogating the human genome continues to evolve, refinement of comparative genomic hybridization (CGH) using array CGH and related technologies have provided enormous insight into human cancers at an affordable scale. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Array Comparative Genomic Hybridization: Protocols and Applications provides researchers with well-honed methodologies to learn these techniques for their own use in research or clinical diagnostic laboratories.