Aqueous Two-Phase Systems: Methods and Protocols

ISBN-13: 9780896035416 / Angielski / Twarda / 2000 / 440 str.

Rajni Hatti-Kaul

Opis

A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-A. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Spis treści

Aqueous Two-Phase Systems: A General Overview, Rajni Hatti-Kaul. Preparation and Characterization of Aqueous Two-Phase Systems: The Phase Diagram, Anita Kaul. Preperation of Aqueous Two-Phase Systems, Daniel Forciniti. Measurement of Some Physical Properties of Aqueous Two-Phase Systems, Donald E. Brooks and Raymond Norris-Jones. Partitioning Techniques: Single-Step Partitioning in Aqueous Two-Phase Systems, Telma Teixeira Franco and Rajni Hatti-Kaul. Partition by Countercurrent Distribution (CCD), Hans-Erik Åkerlund. Liquid-Liquid Partition Chromatography (LLPC), Ulla-Britt Hansson and Christer Wingren. Partitioning of Soluble Molecules and Particulates: Metal Ion Separations in Aqueous Biphasic Systems and with ABECTM Resins, Jonathan G. Huddleston, Scott T. Griffin, Jianhua Zhang Heather D. Willauer, and Robin D. Rogers. Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems, I-Ming Chu and Wen-Yi Chen. Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity, Mark A. Eiteman. Eukaryotic Cell Partition: Experimental Considerations, James M. Van Alstine. Concentration and Purification of Viruses, Lena Hammar. Isolation of Plant Plasma Membranes and Production of Inside-Out Vesicles, Christer Larsson and Susanne Widell. Isolation of Inside-Out Thylakoid Vesicles, Hans-Erik Åkerlund. Two-Phase Partitioning as a Method for Isolation of Tight Plasma Membrane Vesicles from Saccharomyces cerevisiae and from Chlamydomonas reinhardtii, Birgitta Norling. Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning, Birgitta Norling. Purification of Plasma Membranes by Affinity Partitioning, Bengt Jergil and Lars Ekblad. Studying the Influence of Salts on Partitioning of Proteins: Isoelectric Point Determination, Daniel Forciniti. Partitioning of Chemically Modified Proteins: A Method for Detection of the Resolution of Aqueous Two-Phase Systems, Telma Teixeira Franco. Detection and Analysis of Interactionsby Two-Phase Partition, Lars Backman. Cryopartitioning in Two-Phase Systems Containing Organic Solvents, Göte Johansson. Isolation and Purification of proteins by Two-Phase Ectraction: Optimization of Extractions in Aqueous Two-Phase Systems, Ulrich Menge. Two-Phase Extraction of Proteins from Cell Debris, Kristina Köhler Van Alstine and Andres Veide. Aqueous Two-Phase Extraction of Proteins from Animal Tissue, Michael J. Boland. Chaotropic Aqueous Two-Phase Systems: Preparation and Uses, Daniel Forciniti. Temperature-Induced Phase Partitioning for Protein Purification, Anita Kaul, Josefine Persson, and Folke Tjerneld. Extraction of Amphiphilic Proteins Using Detergent-Based Aqueous Two-Phase Systems, Torsten Minuth. Use of Charged PEG and Dextran Derivatives for Biomolecule Partitioning, Göte Johansson. Affinity Partitioning Using Poly(ethylene glycol) with Covalently Coupled Hydrophobic Groups, Vithaldas P. Shanbhag and Poul Erik H. Jensen. Dye-Ligand Affinity Partitioning of Proteins, Gerhard Kopperschläger and Jürgen Kirchberger. Metal Affinity Protein Partitioning, Roberto Z. Guzmán and Javier E. García. Recovery of Proteins and Phase Components, Göte Johansson. Combination of Extraction with Adsorption for Protein Purification, Gabriel Raya-Tonetti and Nora I. Perotti. Integration of Extraction with Affinity Precipitation, Masamichi Kamihira, Rajni Hatti-Kaul, and Bo Mattiasson. Affinity Partitioning Using Magnetic Two-Phase Systems, Masamichi Kamihira. Large-Scale Extraction of Proteins, Teresa Cunha and Raquel Aires-Barros. Bioconversions in Two-Phase Systems: Extractive Bioconversion in Aqueous Two-Phase Systems, Rajni Hatti-Kaul. Enzyme Reaction in Polymer-Polymer Organic Solvent Two-Phase System, Bo Mattiasson. Index.

Blurb

In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented.
Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.