Biophysical Characterization of Aptamer-Target Interactions.- Impedimetric Aptamer-based Biosensors: Principles and Techniques.- Impedimetric Aptamer-based Biosensors: Applications.- Aptamer-based affinity chromatography for protein extraction and purification.- Aptamers in diagnostic and molecular imaging applications.- Aptamer-modified nanoparticles in medical applications.- Defining Target Product Profiles (TPPs) for Aptamer based diagnostics.

This book reviews the development, characterization and applications of aptamers in different areas of biotechnology ranging from therapeutics to diagnostics and protein purification. Hailed as chemical antibodies, these single-stranded nucleic acid receptors were predicted to supersede antibodies in traditional assays, such as ELISA, within a short time. While this has yet to happen, readers will find in this book a deep insight into the progress of aptamer technology and a critical discussion about the limitations that need to be overcome in order to find wider acceptance and use outside of the still relatively small aptamer-community.

This book covers all aspects of aptamer generation and application for the aptamer-experienced reader and curious novice alike, with the addition of an industry perspective on the future of aptamer-use in biotechnology.

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