Antifungal Agents

ISBN-13: 9781588292773 / Angielski / Twarda / 2005 / 210 str.

Erika J. Ernst; Erika J. Ernst

Opis

A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists and molecular biologists the productive tools they need today to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.

Recenzje

From the reviews:

"Due to the increased prevalence in systemic fungal infections worldwide, more knowledge is needed about antifungal drug testing and research is very important. This book, which is one in the series of 'Methods in Molecular Medicine', describes both traditional and new techniques for the investigation of antifungal resistance and for the development and evaluation of new compounds. ... By and large, this is an interesting book for mycological researchers in both laboratory and clinical medicine." (Prof. Jan Faergemann, Acta Dermato-Venereologica, Vol. 86, August, 2006)

Spis treści

Part I. Introduction Susceptibility Testing Methods of Antifungal Agents Erika J. Ernst Part II. Antifungal Resistance Deoxyribonucleic Acid Fingerprinting Methods for Candida Species Shawn R. Lockhart, Claude Pujol, Andrew R. Dodgson, and David R. Soll CARE-2 Fingerprinting of Candida albicans Isolates Joachim Morschhäuser, Peter Staib, and Gerwald Köhler Targeted Gene Deletion in Candida albicans Wild-Type Strains by MPAR Flipping Joachim Morschhäuser, Peter Staib, and Gerwald Köhler Application of Deoxyribonucleic Acid Microarray Analysis to the Study of Azole Antifungal Resistance in Candida albicans Katherine S. Barker and P. David Rogers Application of Proteomic Analysis to the Study of Azole Antifungal Resistance in Candida albicans Massoumeh Z. Hooshdaran, George M. Hilliard, and P. David Rogers Techniques for Antifungal Susceptibility Testing of Candida albicans Biofilms Gordon Ramage and José Luis López-Ribot Part III. Antifungal Development and Evaluation Natural Products and Antifungal Drug Discovery Melissa R. Jacob and Larry A. Walker Use of an Animal Model of Disseminated Candidiasis in the Evaluation of Antifungal Therapy David Andes Murine Model of Invasive Aspergillosis Russell E. Lewis and Nathan P. Wiederhold Antifungal Combinations Roxana G. Vitale, Javier Afeltra, and Eric Dannaoui Post-Antifungal Effect Methods Roxana G. Vitale Part IV. Host Responses and Immunotherapy Evaluation of Immunotherapy in Invasive Candidiasis: Antifungal Activity and Cytokine Expression Assays Maria Simitsopoulou and Emmanuel Roilides Interaction of Histoplasma capsulatum With Human Macrophages, Dendritic Cells, and Neutrophils Simon L. Newman Immunology of Infection Caused by Cryptococcusneoformans Floyd L. Wormley, Jr. and John R. Perfect Index

Blurb

With the growing frequency of fungal infections even as resistance to existing antifungal agents increases, methods for finding novel drug targets and evaluating antifungal resistance have become more important than ever. In Antifungal Agents: Methods and Protocols, expert scientists describe in detail the state-of-the-art molecular methods they have optimized for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. Many of the protocols were developed in the laboratory and, when a method is limited to specific organisms, include alternative techniques to expand their application. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists, and molecular biologists the productive tools they need today in order to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.