Animal Endo-Sirnas: Methods and Protocols

ISBN-13: 9781493941605 / Angielski / Miękka / 2016 / 182 str.

Andreas Werner

Opis

Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs. These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.

Spis treści

1. Targeted Small Non-coding RNA Directed Gene Activation in Human Cells

Caio Damski and Kevin V. Morris

 

2. Isolation of Chromatoid Dodies from Mouse Testis as a Rich Source of Short RNAs

Oliver Meikar and Noora Kotaja

 

3. Generation of Endo-siRNAs in Xenopus laevis Oocytes

Sammer Alnumeir and Andreas Werner

 

4. Analysis of Endo-siRNAs in Drosophila

Katharina Elmer, Stephanie Helfer , Milijana Mirkovic-Hösle and Klaus Förstemann

 

5. Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster

Do-Hwan Lim, Chun-Taek Oh, Sung-Jun Han, and Young Sik Lee

 

6. Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans

Maria C. Ow, Nelson C. Lau, and Sarah E. Hall

 

7. Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs

Philip K. Shiu, Jimmy J. Zhuang, and Craig P. Hunter

 

8. Extraction and Non-radioactive Detection of Small RNA Molecules

Mark Carlile and Andreas Werner

 

9. p19 Mediated Enrichment and Detection of siRNAs

Jingmin Jin, Larry A. McReynolds^, and Monika Gullerova

 

10. Detection of Small non-coding RNAs by in situ Hybridization using Probes of 2’-O-Methyl RNA + LNA

Martin Jensen Søe, Martin Dufva, and Kim Holmstrøm

 

11. Enhanced Detection of Small RNAs using a non-Radioactive Approach

Teresa T. Liu, Zhihua Li, and  Bino John

 

12. Computing siRNA and piRNA Overlap Signatures

Christophe Antoniewski

 

13. Isolation of Small Interfering RNAs using Viral Suppressors of RNA Interference

Marius van den Beek, Christophe Antoniewski and Clément Carré

 

14. Computational Analysis, Biochemical Purification, and Detection of tRNA-derived Small RNA Fragments

Simon P. Keam, Andrew Sobala, David T. Humphreys, Catherine M. Suter, and Gyorgy Hutvagner

15. Differential DNA Methylation Patters in Endo-siRNA Mediated Silencing of LINE-1 Retrotranspons

Long Chen, Jane E Dahlstrom, and Danny Rangasamy