Analytical Ultracentrifugation IV

ISBN-13: 9783662160503 / Angielski / Miękka / 2013 / 194 str.

Rainer Jaenicke; Helmut Durchschlag

Opis

This volume contains the contributions of the 10th Symposium on Analytical Ultracentrifugation, held in Regensburg, Germany, on March 13th to 14th, 1997. Topics in the following fields were presented: Technical innovations; innovations in data analysis; modeling; biological systems; ions and polyelectrolytes; polymers, colloids, supramolecular systems; emulsions, gels, and dispersions; interacting systems and assemblies.

Spis treści

Direct analysis of sedimentation equilibrium distributions reflecting macromolecular interactions.- Alternative strategies for the characterization of associations in multicomponent solutions via measurement of sedimentation equilibrium.- Non-linear curve-fitting methods for data from the XL-A analytical utltracentrifuge.- Rapid molecular mass determination by sedimentation velocity experiments and direct fitting of the concentration profiles.- A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macromolecular interactions.- Calculation of hydrodynamic parameters of biopolymers from scattering data using whole-body approaches.- Calculation of hydrodynamic parameters of proteins from crystallographic data using multibody approaches.- Analytical ultracentrifugation with fluorescence detection and biosafety containment and its application to the prion protein.- Dye-labelling as a means to study ternary protein complexes by analytical ultracentrifugation: the band 3/ankyrin/aldolase complex from erythrocyte membranes.- Studies of ligand-mediated conformational changes in enzymes by difference sedimentation velocity in the Optima XL-A ultracentrifuge.- Quaternary structure and interaction parameters of bovine ?-crystallin: influence of isolation conditions.- Investigation of irradiated eye-lens proteins by analytical ultracentrifugation and other techniques.- Sedimentation analysis of SDS and albumin-SDS complexes.- The self-association of basic helix-loop-helix peptides.- Nuclear DNA fractions with grossly different base ratios in the genome of the marine sponge Geodia cydonium.- Sedimentation equilibrium studies of synthetic polyelectrolytes by means of interference optical methods.- Investigation of quantum size colloids using the XL-I ultracentrifuge.- Influence of pressure and solvent composition on the density gradient in the analytical ultracentrifuge I extended Hermans-Ende equation for the equilibrium density gradient.- Influence of pressure and solvent composition on the density gradient in the analytical ultracentrifuge. II direct refractometric determination of the equilibrium and non-equilibrium density gradient.- First experiences with the new XL-I AUC: Applications in polymer and colloid science.- Analytical ultracentrifugation as a tool in supramolecular chemistry: a feasibility study using a metal coordination array.- The sedimentation velocity of a gelled polymer.- New contributions of analytical ultracentrifugation to the investigation of dispersions.- Determination of hydrodynamic radius: a comparison of ultracentrifuge methods with dynamic light scattering.