Analytical Ultracentrifugation

ISBN-13: 9783662156025 / Angielski / Miękka / 2013 / 210 str.

Joachim Behlke

Opis

The 9th Symposium on Analytical Ultracentrifugation (AUC) which was held at the Max Delbruck Center for Molecular Medicine in Berlin-Buch, March, 1995, is the basis of this volume. It presents an actual survey of recent developments and results of ultracentrifugation methods in the frame of the following topics: Reversible Association Reactions.- Analysis of Supramolecular Structures Including Modeling.- Size Distribution Analysis and Gel Formation.- Hardware and Software Developments.

Spis treści

Self-association of the molecular chaperone HSC70 as assessed by analytical ultracentrifugation.- Investigation of the molecular chaperone DnaJ by analytical ultracentrifugation.- Investigations of the oligometric state of the 42 kDa repressor isoform from the streptomcyes temperate bacteriophage ?C31.- Characterisation of the solution structure of halophilic proteins. Analytical centrifugation among complementary techniques (light, neutron and X-ray scattering, density measurements).- ?-helical coiled coils: simple models for self-associating peptide and protein systemss.- Improved approach for characterizing the coalescence stability of legumin stabilized O/W emulsions by analytical ultracentrifugation.- Preliminary ultracentrifuge studies of the polyelectrolyte behaviour of Welan gum.- Netropsin-induced changes of DNA supercoiling; sedimentation studies.- Sedimentation studies of specific association of oligonucleosomes from sea urchin sperm chromatin.- Complex formation and crystallization of adrenodoxin-reeuctase with adrenodoxin.- Studying heterologous associations between membrane proteins by analytical ultracentrifugation: Experience with erythrocyte band 3.- Chemical cross-linking and analytical ultracentrifugation study of the histone-like protein HBsu: Quaternary structure and DNA binding.- Hydrodynamic modelling of the solution conformation of 10 S myosin.- Supramolecular structure of the small heat shock protein Hsp25.- Physical-chemical characterization of the different individual cortical alfa-crystallin fractions from bovine lenses.- Sedimentation parameter of linear polymers.- Velocity sedimentation of water-soluble methyl cellulose.- Simultaneous determination of particle and density distributions of dispersions by analytical ultracentrifugation.- Molar mass distribution of polymers from sedimentation velocity in an analytical ultracentrifuge.- Molar mass distribution from sedimentation fractiometry (SF)-competition with size exclusion chromatography (SEC, GPC).- Reaction kinetic and molar mass distribution of the polycation poly[(dimethyleneimino)ethylene-(dimethyleneimino)-methylene-1,4-phenylenemethylenedichloride].- Direct digital data capture for sedimentation velocity experifments using UV/VIS optics.- Studies of microgel formation in aqueous and organic solvents by light scattering and analytical ultracentrifugation.- Formation of reversible concentration gradients during the centrifugation of gels.- Measurement of the extinction coefficient of prostate specific antigen using interference and absorbance optics in the Optima XL-A analytical ultracentrifuge.- A study on Schlieren patterns derived with the Beckman Optima XL-A UV-absorption optics.- A comparative “Schlieren” study of the sedimentation behaviour of three polysaccharides using the Beckman Optima XL-A and model E analytical ultracentrifuges.- Alternative light sources for the Schlieren optical system of analytical ultracentrifuges.- The determination of liquid/liquid interfacial mass transfer A new application for the analytical ultracentrifuge.