Analysis of Post-Translational Modifications and Proteolysis in Neuroscience

ISBN-13: 9781493934706 / Angielski / Twarda / 2016 / 290 str.

Jennifer Elizabeth Grant; Hong Li

Opis

This volume highlights proteomics studies of quantitative PTM changes in both peripheral and central nervous system proteomes utilizing the most recent advances in mass spectrometry. Chapters include practical information pertaining to the fundamentals of sample preparation, liquid chromatography, and tandem mass spectrometry instrumental analysis and will elucidate best practices in the interpretation of data using modern bioinformatics approaches. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical, Analysis of Post-Translational Modifications and Proteolysis in Neuroscience aims to ensure successful results in the further study of this vital field.

Spis treści

1. Proteomic Analysis of Post-Translational Modifications in Neurobiology

Hongbo Gu, Matthew P. Stokes, and Jeffrey C. Silva

 

2.  A Boronic Acid-based Enrichment for Site-specific Identification of the N-glycoproteome using MS-based Proteomics

Haopeng Xiao, George X. Tang, Weixuan Chen, and Ronghu Wu

 

3. Site-specific Localization of D-Amino Acids in Bioactive Peptides by Ion Mobility Spectrometry

Chenxi Jia, Christopher B. Lietz, Qing Yu, and Lingjun Li

 

4. Quantitative Profiling of Reversible Cysteome Modification under Nitrosative Stress

Yue-Ting Wang, Sujeewa C. Piyankarage, and Gregory R. J. Thatcher

 

5. Label-free LC-MS/MS Comparative Analysis of Protein S-nitrosome in Synaptosomes from Wild Type and APP Transgenic Mice

Monika Zaręba-Kozioł, Maciej Lalowski, and Aleksandra Wysłouch-Cieszyńska

 

6. Proteomics Identification of Redox-Sensitive Nuclear Protein Targets Of Human Thioredoxin 1 from  SHSY-5Y Neuroblastoma Cell Line

Mohit Raja Jain, Changgong Wu, Qing Li, Chuanlong Cui, Huacheng Dai, and Hong Li

 

7. Identification and Quantification of K63-Ubiquitinated Proteins in Neuronal Cells by Hi

gh-

Resolution Mass Spectrometry

Gustavo Monteiro Silva, Wei Wei, Sandhya Manohar,  and Christine Vogel

 

8.  Phosphorylation Site Profiling of NG108 Cells Using Quadrupole/Orbitrap Mass

Spectrometry

Fang-Ke Huang, Guoan Zhang, and Thomas A. Neubert

 

 9. Phosphoproteomics of Tyrosine Kinases in the Nervous System

Robert J. Chalkley and

Ralph A. Bradshaw

 

10. Analysis of PINK1 and CaMKII Substrates Using Mass Spectrometry-Based Proteomics

Yan Li, Lesley A. Kane, Michael A Bemben, and Katherine W Roche

 

11. Liquid Chromatography-Tandem Mass Spectrometry Analysis of Tau Phosphorylation

Jhoana Mendoza,Georgia Dolios, and Rong Wang

 

12. Identification of Protease Substrates in Complex Proteomes by iTRAQ-TAILS

Tobias Kockmann, Nathalie Carte, Samu Melkko, and Ulrich auf dem Keller

 

13. Proteolytic P

rocessing of Neuropeptides

 

Lloyd D. Fricker

 

14 Isotope Dilution Analysis of Myelin Basic Protein Degradation after Brain Injury

Andrew K Ottens

 

15. Identification and Characterization of Protein Post-Translational Modifications by

Differential Fluorescent Labeling

Eric D. Hamlett, Cristina Osorio, and Oscar Alzate

 

16 Mass

Spectrometric Detection of Detyrosination and Polyglutamyation on the C-Terminal Region of Brain Tubulin

Yasuko Mori, Alu Konno, Mitsutoshi Setou, and Koji Ikegami

 

17. Determination of polyhydroxybutyrate (PHB) Post-Translational Modifications of Proteins Using Mass Spectrometry

Tong Liu, Wei Chen, Stacey Pan, Chuanlong Cui, Hong Li, and Eleonora Zakharian