Amino Acid Analysis: Methods and Protocols

ISBN-13: 9781493996414 / Angielski / Miękka / 2020 / 455 str.

Michail A. Alterman

Spis treści

Dedication…

Preface…

Table of Contents…

Contributing Authors…

1. Ultra-Sensitive Quantification of Recombinant Proteins using AAA-MS

Mathilde Louwagie, Sylvie Kieffer-Jaquinod, and Virginie Brun

2.   Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry

Olga A. Mirgorodskaya, Roman Körner, Yuri P. Kozmin, and Peter Roepstorff

3.   Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometry

Natalia V. Gogichaeva and Michail A. Alterman

4. Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content

Michelle L. Colgrave, Peter G. Allingham, Kerri Tyrrell, and Alun Jones

5. Analysis of Hydroxyproline in Collagen Hydrolysates

Tobias Langrock and Ralf Hoffmann

6. Quantification of Isotopologues of Amino Acids by Multiplexed Stable Isotope Resolved Metabolomics using Ultrahigh Resolution Mass Spectrometry Coupled with Direct Infusion

Ye Yang, Teresa W.M. Fan, Andrew N. Lane, and Richard M. Higashi

7. Preparative HPLC Separation of Underivatized Amino Acids for Isotopic Analysis

Jennifer A. Tripp, Thibaut Devièse, and James S. O. McCullagh

8. Quantitative Analysis of Underivatized Amino Acids by Liquid Chromatography-Tandem Mass Spectrometry

Justin Mak, Tina M. Cowan, and Anthony Le

9. Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spectrometry

Megumi Kato and Akiko Takatsu

10. The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determination of Plasma Tryptophan and other Amino Acids

Abdulla A.-B. Badawy

11. Analysis of Tryptophan and Its Metabolites by High-Performance Liquid Chromatography

Zhaolai Dai, Shiqiang Sun, Hui Chen, Moyan Liu, Lianhua Zhang, Zhenlong Wu, Ju Li, and Guoyao Wu

12. Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis

David M. Bunk and Mark S. Lowenthal

13. Quantification of Underivatized Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC-ESI-MS/MS

Giuseppe Giordano, Antonina Gucciardi, Paola Pirillo, and Mauro Naturale

14. Amino Acid Analysis in Physiological Samples by GC-MS with Propylchloroformate Derivatization and iTRAQ-LC-MS/MS 

Katja Dettmer, Axel P. Stevens, Stephan R. Fagerer, Hannelore Kaspar, and Peter J. Oefner

15. Combination of an AccQ·Tag-Ultra Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino Acids

Carolina Salazar, Jenny M. Armenta, Diego F. Cortes, and Vladimir Shulaev

16. Methods for Absolute Quantification of Human Plasma Free Amino Acids by High–Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry using Precolumn Derivatization

Akira Nakayama, Akira Imaizumi, and Hiroo Yoshida

17. Analysis and Enantioseparation of Amino Acids by Liquid Chromatography

Poonam Malik and Ravi Bhushan

18. Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Chiral and Nonchiral Amino Acid Analysis by Gas Chromatography

Petr Šimek, Petr Hušek and Helena Zahradníčková

19. High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis without Derivatization  

Yosuke Nakano, Moyu Taniguchi, Yutaka Umakoshi, Daisuke Watai, and Eiichiro Fukusaki

20. A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids

Sonja Hess

21. Chiral Analysis of Non-Protein Amino Acids by Capillary Electrophoresis

María Castro-Puyana and María Luisa Marina 

22. Amino Acid Analysis via LC–MS Method after Derivatization with Quaternary Phosphonium

Shinsuke Inagaki and Toshimasa Toyo’oka

23. Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry

Akiyoshi Hirayama, Satsuki Ikeda, Asako Sato, and Tomoyoshi Soga

24. Capillary Electrophoresis of Free Amino Acids in Physiological Fluids without Derivatization Employing Direct or Indirect Absorbance Detection

Gordana D. Žunić, Slavica Spasić, and Zorana Jelić-Ivanović

25. New Advances in Amino Acid Profiling in Biological Samples by Capillary Electrophoresis-Mass Spectrometry

Meera Shanmuganathan and Philip Britz-McKibbin

26. Enhanced Separation Capability of Sequential Injection Chromatography for Fluorimetric Determination of Intracellular Dissolved Free Amino Acids in Marine Microalgae

Marilda Rigobello-Masini and Jorge C. Masini

27. Sensitive and Selective Amino Acid Profiling of Minute Tissue Amounts by HPLC/Electrospray Negative Tandem Mass Spectrometry using 9-Fluorenylmethoxycarbonly (Fmoc-Cl) Derivatization

Jörg Ziegler, Hina Hussain, Reinard H.H. Neubert, and Steffen Abel

28. Wheat Gluten Amino Acid Analysis by High-Performance Anion-Exchange Chromatography with Integrated Pulsed Amperometric Detection

Ine Rombouts, Bert Lagrain, Lieve Lamberts, Inge Celus, Kristof Brijs, and Jan A. Delcour

29. Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass Spectrometry

Madeleine Dell’mour, Gunda Koellensperger, and Stephan Hann

30. Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS-MS (Improved Method)

Björn Thiele, Michelle Hupert, Beatrix Santiago-Schübel, Marco Oldiges, and Diana Hofmann

31. Optimal Conditions for the Direct RP-HPLC Determination of Underivatized Amino Acids with On-Line Multiple Detection 

A. Pappa-Louisi, P. Agrafiotou, and S. Sotiropoulos

32. Non-Marking Collection of Amino Acids from Fingerprints using Hydrogels

Ward van Helmond, Vincent O'Brien, Robin de Jong, Jan van Esch, Sander Oldenhof, and Marcel de Puit

 

33. Amino Acid Profiling from Fingerprints using Amide Stationary Phase UPLC-MS

Ward van Helmond and Marcel de Puit 

Blurb

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.