Allergy Methods and Protocols

ISBN-13: 9781617372438 / Angielski / Miękka / 2010 / 352 str.

Meinir G. Jones; Penny Lympany

Opis

Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.

Recenzje

From the reviews:

"The purpose is to provide a comprehensive and readily accessible guide to techniques used in the investigation of allergic diseases. ... Students embarking on an investigative career in allergy and immunology will find this book particularly useful. ... would find a ready place in most laboratories where postgraduate students are working. ... It can be highly recommended to researchers who are starting out, and to those who would like an authoritative and succinct background to their work." (Ronald S. Walls, Doody's Review Service, October, 2008)

Spis treści

List of chapters 1. Understanding the molecular mechanisms of allergy - Meinir Jones 2. T cell - primary culture from peripheral blood - Monika Raulf-Heimsoth 3. Production of T cell lines - Helga Kahlert 4. Production of human T cell clones - Adrienne Verhoef 5. Mapping of human T-cell epitopes of allergens - Thomas Zeiler and Tuomas Virtanen 6. Determining MHC restriction of T cell response - Mark Larche 7. Short term culture of CD8 cells and intracellular cytokine staining - Beejal Vyas and Alistair Noble 8. Isolation, flow cytometric analysis and suppression assay of CD4+CD25+ T regulatory cells - Hayley Jeal 9. Monocyte-derived dendritic cells as antigen presenting cells in T cell proliferation and cytokine production - Sun-sang J Sung 10. Ultrasensitive ELISA for measurement of human cytokine responses in primary culture - William P. Stefura, J. Darren Campbell, Renée Douville, Monique J. Stinson, F. Estelle Simons, Allan B. Becker, and Kent T. HayGlass 11. Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses - Monique Stinson, Renee Douville, Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons, Allan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass 12. Standardisation of allergen extracts - Jørgen Nedergaard Larsen and Sten Dreborg 13. Immuno-electrophoresis for the characterisation of allergen extracts - Gitte Nordskov Hansen and Jørgen Nedergaard Larsen 14. Conjugation of haptens - Ranulfo Lemus and Meryl H. Karol 15. Monoclonal antibodies - Helga Kahlert and Oliver Cromwell 16. Purification of antibodies - Per H Larsson and Anne Renström 17. Collection of air samples to quantify exposure to airborne allergens - Susan Gordon 18. Assay of air sample eluates - Anne Renström and Susan Gordon 19. The halogen assay - a new technique for measuring airborne allergen - Euan Tovey,Sandra De Lucca, Leanne Poulos and Tim O'Meara 20. Measurement of specific IgG anti-Fel d 1 antibodies - Meinir Jones 21. The facilitated-antigen binding (FAB) assay - a protocol to measure allergen-specific inhibitory activity - James N Francis 22. Microscopic identification and purity determination of pollen grains - Magdalena Rahl 23. Biopanning for the characterisation of allergen mimotopes - Isabella Schöll and Erika Jensen-Jarolim 24. Identification of mast cells and mast cell subpopulations - Mark Buckley and Andrew F Walls 25. Purification and characterisation of mast cell tryptase and chymase - Alan R McEuen and Andrew F Walls 26. Experimental activation of mast cells and their pharmacological modulation - Shaoheng He and Andrew F Walls 27. In situ hybridisation - Kayhan T. Nouri-Aria

Blurb

In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Protocols a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of protocols. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation. Allergy: Methods and Protocols will be a remarkably useful bench tool for anyone embarking in or continuing with their research in allergy.