Advances in Plant Ecophysiology Techniques

ISBN-13: 9783030066178 / Angielski / Miękka / 2018 / 497 str.

Adela M. Sanchez-Moreiras; Manuel J. Reigosa

Spis treści

Preface

1. In vitro and in vivo bioassays
2. Root morphology
3. Plant photosynthetic pigments: Methods and tricks for correct quantification and identification
4. Measuring photosynthesis and respiration with infrared gas analysers
5. Chlorophyll fluorescence: a practical approach to study ecophysiology of green plants
6. Thermoluminescence: a tool to study ecophysiology of green plants
7. Determining plant water relations
8. Thermal imaging and infrared sensing in plant ecophysiology
9. Photoprotection and photo-oxidative stress markers as useful tools to unravel plant invasion success
10. Reactive oxygen species and antioxidant enzymatic systems in plants: role and methods
11. Flow cytometric measurement of different physiological parameters
12. Flow cytometry: cell cycle
13. Mitotic index
14. Fluorescent probes and live imaging of plant cells
15. Confocal and transmission electron microscopy for plant studies..
16. Plant programmed cell death (PCD): using cell morphology as a tool to investigate plant PCD
17. Visualization of plant microtubules
18. Multiprobe in-situ hybridization to whole mount Arabidopsis seedlings
19. Proteomics analysis of plant tissues based on two-dimensional gel electrophoresis
20. Metabolomics and metabolic profiling – investigation of dynamic plant-environment interactions at the functional level
21. SAR / QSAR
22. Elucidating the phytotoxic potential of natural compounds
23. Exploring plants strategies for allelochemical detoxification
24. Chemical characterization of volatile organic compounds (VOCs) through headspace solid phase micro extraction (SPME)
25. Carbon radiochemicals (¹⁴C) and stable isotopes (¹³C): crucial tools to study plant-soil interactions in ecosystems
26. Stable-isotope techniques to investigate sources of plant water
27. Soil microorganisms
28. Computational approach to study ecophysiology

Blurb

This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death, and other parameters; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.

Every chapter is focused on the measurement of a parameter from a very practical point of view, including its use in plant ecophysiology and the meaning of the results that can be obtained.