Advanced Protocols in Oxidative Stress III

ISBN-13: 9781493914401 / Angielski / Twarda / 2014 / 477 str.

Donald Armstrong

Opis

Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.

Spis treści

Part I: Reactive Oxygen and  Nitrogen Techniques

 

1. Derivatization and Detection of Small Aliphatic and Lipid-Bound Carbonylated Lipid Peroxidation Products by ESI-MS

            Ivana Milic and Maria Fedorova

 

2. Specific Imaging and Tracking of Mitochondria in Live Cells by a Photostable AIE Luminogen

            Chris W.T. Leung, Yuning Hong, and Ben Zhong Tang

 

3. Analysis of Relationship Between Oxidized Phospholipid Structure and Interaction with the Class B Scavenger Receptors

            Detao Gao, Larry W. Sayre, and Eugene A. Podrez

 

4. A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA

            Takayuki Shibamoto

 

5. Nitric Oxide Availability as a Marker of Oxidative Stress

            Dan Pierini and Nathan S. Bryan

 

6. Quantification of DNA Repair Capacity Towards Oxidatively Damaged DNA in Subcellular and Cellular Systems by a Non-Radioactive Cleavage Assay

            Ingrit Hamann and Andrea Hartwig

 

7. Chiral Phase-HPLC Separation of Hydroperoxyoctadecenoic Acids and Their Biosynthesis by Fatty Acid Dioxygenases

            Ernst H. Oliw and Anneli Wennman

 

8. Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and H₂S Repair

            Zhangrong Lou, Peng Li, and Keli Han

 

9. Evaluation of Physical Integrity of Lipid Bilayer Under Oxidative Stress: Application of Fluorescence Microscopy and Digital Image Processing

            Ran Liang, Jian-Ping Zhang, and Leif H. Skibsted

 

10. Electrochemical Detection of Glutathione S-Transferase: An Important Enzyme in the Cell Protective Mechanism Against Oxidative Stress

            Manuel C. Martos-Maldonado, Juan M. Casas-Solvas, Antonio Vargas-Berenguel, and Luis García-Fuentes

 

11. Using Nanosensors for In Situ Monitoring and Measurement of Nitric Oxide and Peroxynitrite in a Single Cell

            Tadeusz Malinski

 

Part II: Antioxidant Technology and Application

 

12. Curcuma longa Attenuates Carbon Tetrachloride-Induced Oxidative Stress in T Lymphocyte Subpopulations

            H.A. Abu-Rizq, Mohamed H. Mansour, and Mohammad Afzal

 

13. Prolongation of the Lag Time Preceding Peroxidation of Serum Lipids: A Measure of Antioxidant Capacity

            Ilya Pinchuk and Dov Lichtenberg

 

14. Identification of (Anti-Oxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements

            Eric Deconinck, Deborah Custers, and Jacques Omer De Beer

 

15. Simultaneous Determination of Ascorbic Acid, Aminothiols, and Methionine in Biological Matrices Using Ion-Pairing RP-HPLC Coupled with Electrochemical Detector

            Muhammad Imran Khan, Zafar Iqbal, and Abad Khan

 

16. Portable Nanoparticle Based Sensors for Antioxidant Analysis

            Erica Sharpe and Silvana Andreescu

 

17. Fe³⁺-Fe²⁺ Transformation Method: An Important Antioxidant Assay

            İlhami Gülçin

 

18. Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity

            Augustin C. Mot, Cristina Bischin, Grigore Damian, and Radu Silaghi-Dumitrescu

 

19. Computational Studies on Conformation, Electron Density Distributions, and Antioxidant Properties of Anthocyanidins

            Ricardo A. Mosquera, Laura Estévez, and Mercedes García-Bugarín

 

20. Automatic Flow Injection Analysis (FIA) Determination of Total Reducing Capacity in Serum and Urine Samples

            Marcela A. Segundo, Ildikó V. Tóth, Luís M. Magalhães, and Salette Reis

 

21. Mass Spectrometry Detection of Isolevuglandin Adduction to Specific Protein Residues

            Casey D. Charvet and Irina A. Pikuleva

 

22. Preoxiredoxin Family Members (Prx3 & Prx4) and Pregnancy Disorder (Recurrent Pregnancy Loss)

            Behrouz Gharesi-Fard

 

23. Substituted Pyridoindoles as Biological Antioxidants: Drug Design, Chemical Synthesis, and Biological Activity

            Lucia Kovacikova, Magdalena Majekova, and Milan Stefek

 

24. Measuring Antioxidant and Prooxidant Capacity Using the Crocin Bleaching Assay (CBA)

            Stella A. Ordoudi and Maria Z. Tsimidou

 

25. Simultaneous Determination of the Endogenous Free α−Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP−HPLC Coupled with Electrochemical Detector

            Muhammad Imran Khan, Zafar Iqbal, and Abad Khan

 

26. Aryl-Acetic and Cinnamic Acids as Lipoxygenase Inhibitors with Antioxidant, Anti-Inflammatory, and Anti-Cancer Activity

            Dimitra Hadjipavlou-Litina and Eleni Pontiki

 

27. Liquid Chromatography and Mass Spectrometry for the Analysis of N-β-Methylamino-L-Alanine with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate

            W. Broc Glover, Steven A. Cohen, and Susan J. Murch

 

Part III: Gene Expression

 

28. Intracellular Distribution of Glutathionylated Proteins in Cultured Dermal Fibroblasts by Immunofluorescence

            Stefania Petrini, Valentina D’Oria, and Fiorella Piemonte

 

29. New Analytical Strategies Applied to the Determination of Coenzyme Q10 in Biological Matrix

            Contin Mario, Flor Sabrina, Martinefski Manuela, Lucangioli Silvia, and Tripodi Valeria

 

30. Binding and Cytotoxic Trafficking of Cholesterol Hydroperoxides by Sterol Carrier Protein-2

            Albert W. Girotti and Tamas Kriska

 

Part IV: Biostatistics

 

31. A Toolkit for Clinical Statisticians to Fix Problems Based on Biomarker Measurements Subject to Instrumental Limitations: From Repeated Measurement Techniques to a Hybrid Pooled-Unpooled Design

            Albert Vexler, Ge Tao, and Xiwei Chen