Adult Stem Cells: Volume 419

ISBN-13: 9780123736505 / Angielski / Twarda / 2006 / 576 str.

Irina Klimanskaya

Opis

This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook" and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional" and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.

• Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
• Presents the latest most innovative technologies
• Addresses therapeutic relevance including FDA compliance and tissue engineering

Spis treści

Section I: Ectoderm

[1]: Neural Stem Cell Isolation and Characterization

[2]: Neural Stem Cells and Their Manipulation

[3]: Retinal Stem Cells

[4]: Epithelial Skin Stem Cells

[5]: Dental Pulp Stem Cells

Section II: Mesoderm

[6]: Postnatal Skeletal Stem Cells

[7]: Hematopoietic Stem Cells

[8]: Hemangioblasts and Their Progeny

[9]: Kidney Epithelial Cells

[10]: Ovarian Germ Cells

[11]: Spermatogonial Stem Cells

Section III: Endoderm

[12]: Stem Cells in the Lung

[13]: Pancreatic Cells and Their Progenitors

[14]: Intestinal Epithelial Stem Cells and Progenitors

Section IV: Extraembryonic and Perinatal Stem Cells

[15]: Trophoblast Stem Cells

[16]: Pluripotent Stem Cells from Germ Cells

[17]: Amniotic Fluid and Placental Stem Cells

[18]: Cord Blood Stem and Progenitor Cells

Author Index

Subject Index

O autorze

Robert Lanza is an American scientist and author whose research spans the range of natural science, from biology to theoretical physics. TIME magazine recognized him as one of the "100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 "World Thinkers.” He has hundreds of scientific publications and over 30 books, including definitive references in the fields of stem cells, tissue engineering, and regenerative medicine. He's a former Fulbright Scholar and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman (known for his work on the biological basis of consciousness) and Rodney Porter. He also worked closely (and co-authored papers in Science on self-awareness and symbolic communication) with noted Harvard psychologist BF Skinner. Dr. Lanza was part of the team that cloned the world's first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans.