Adoptive Immunotherapy: Methods and Protocols

ISBN-13: 9781617375460 / Angielski / Miękka / 2010 / 500 str.

Burkhard Ludewig; Matthias W. Hoffmann

Opis

An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Recenzje

"...a comprehensive and authoritative collection of currently used protocols for adoptive immunotherapy in both pre-clinical and clinical settings." - Immunology News

"...will be not only useful for basic researchers but also clinical scientists aiming to translate basic research to a clinical setting." - Immunology and Cell Biology

Spis treści

Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection David O'Neill and Nina Bhardwaj A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy Gennady Bocharov, Neville J. Ford, and Burkhard Ludewig Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres Ying Waeckerle-Men, Bruno Gander, and Marcus Groettrup RNA Transfection of Dendritic Cells Frank Grünebach, Martin R. Müller, and Peter Brossart Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System John D. M. Campbell, Christoph Piechaczek, Gregor Winkels, Edith Schwamborn, Daniela Micheli, Sonja Hennemann, and Jürgen Schmitz Generation of Clinical Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System Thomas Putz, Hubert Gander, Reinhold Ramoner, Claudia Zelle-Rieser, Andrea Rahm, Walter Nussbaumer, Georg Bartsch, Lorenz Höltl, and Martin Thurnher Adenoviral Transduction of Dendritic Cells Rienk Offringa, Kitty Kwappenberg, Martijn Rabelink, Delphine Rea, and Rob Hoeben Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy David O'Neill and Nina Bhardwaj Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells I. Jolanda M. de Vries, Gosse J. Adema, Cornelis J. A. Punt, and Carl G. Figdor Dendritic Cells in Clinical Trials for Multiple Myeloma Volker L. Reichardt and Peter Brossart Identification of Tumor-Associated Autoantigens With SEREX Özlem Türeci, Dirk Usener, Sandra Schneider, and Ugur Sahin Application of Proteomics and Protein Analysis for Biomarker and Target Finding for Immunotherapy Petra Weingarten, Petra Lutter, Andreas Wattenberg, Martin Blueggel, Sonja Bailey, Joachim Klose, Helmut E. Meyer, and Christoph Huels Isolationand Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy Helga Bernhard, Burkhard Schmidt, Dirk H. Busch, and Christian Peschel Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers Norbert Meidenbauer and Andreas Mackensen Gene Transfer of MHC-Restricted Receptors Helmut W. H. G. Kessels, Monika C. Wolkers, and Ton N. M. Schumacher Immunotherapy With CTL Restricted by Nonself MHC Liquan Gao, Anne-Marie Downs, and Hans J. Stauss Designing TCR for Cancer Immunotherapy Ralf-Holger Voss, Jürgen Kuball, Matthias Theobald Isolation and Expansion of Tumor-Specific CD4+ T-Cells by Means of Cytokine Secretion Christian Becker Methods for the Ex Vivo Characterization of Human CD8+ T Subsets Based on Gene Expression and Replicative History Analysis Nathalie Rufer, Patrick Reichenbach, and Pedro Romero Regulatory T Cells in Antitumor Therapy: Isolation and Functional Testing of CD4+CD25+ Regulatory T-Cells Helmut Jonuleit and Edgar Schmitt Monoclonal Antibody-Based Strategies in Autoimmunity and Transplantation Lucienne Chatenoud Producing Bispecific and Bifunctional Antibodies Dipankar Das and Mavanur R. Suresh Antibody-Cytokine Fusion Proteins for the Therapy of Cancer Gustavo Helguera and Manuel L. Penichet Synthesis and Biological Evaluation of a Paclitaxel Immunoconjugate Ahmad Safavy and Kevin P. Raisch Cytotoxic Tumor Targeting With scFv Antibody-Modified Liposomes Cornelia Marty and Reto A. Schwendener Intravenous Immunoglobin Treatment for Fibrosis, Atherosclerosis, and Malignant Conditions Ilan Krause and Yehuda Shoenfeld Study of T-Cell Costimulatory Blockade In Vivo at a Single Cell Level Minh Diem Vu and Xian Chang Li Stem Cell Transplantation: Graft-Mediated Antileukemia

Blurb

Over the last decade, major advances in our understanding of the immune system and its mechanisms have led to promising new treatment strategies. In Adoptive Immunotherapy: Methods and Protocols, leading experts describe in detail their optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T-cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immonotherapy, and the utilization of regulatory T-cells. Theoretical overviews and discussion of future directions for the improvement of adoptive immunotherapy complete the volume. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Adoptive Immunotherapy: Methods and Protocols offers an authoritative collection of novel techniques to study the adoptive transfer of tailored antigen-specific immune cells and to improve their clinical application.