Adhesion Protein Protocols

ISBN-13: 9781493963119 / Angielski / Miękka / 2016 / 407 str.

Amanda S. Coutts

Opis

This book covers techniques in cellular adhesion. It features lists of the necessary materials and reagents; step-by-step, readily reproducible protocols; and notes on troubleshooting and avoiding known pitfalls.

Spis treści

1. Reconstructing Integrin Activation in vitro

Alexandre R. Gingras, Feng Ye, and Mark H. Ginsberg

 

2. Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy

Jens Friedrichs, Carsten Werner, and Daniel J. Muller

 

3. A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics

Warwick S. Nesbitt, Francisco J. Tovar-Lopez, Erik Westein, Ian S. Harper, and Shaun P. Jackson

 

4. Localization-Based Super-Resolution Imaging of Cellular Structures

Pakorn Kanachanwong and Clare M. Waterman

 

5. Use of Microarray Analysis to Investigate EMT Gene Signatures

Andy H. Sims, A.A. Larionov, D.J. Harrison, and Elad Katz

 

6.  Podosome Reformation in Macrophages: Assays and Analysis

Pasquale Cervero, Linda Panzer, and Stefan Linder

 

7. Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells

Mirjam Andreasson-Ochsner and Erik Reimhult

 

8. Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay

Marie Schoumacher, Alexandros Glentis, Vasily V. Gurchenkov, and Danijela M. Vignjevic

 

9. Cytoplasmic Actin: Purification and Single Molecule Assembly Assays

Scott D. Hansen, J. Bradley Zuchero, and R. Dyche Mullins

 

10. Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity

Aron Parekh,Jonathan M. Page, Scott A. Guelcher, and Alissa M. Weaver

 

11. Determining Rho GTPase Activity by an Affinity-Precipitation Assay

Narendra Suryavanshi and Anne J. Ridley

 

12. Proteomic and Biochemical Methods to Study the Cytoskeletome

Richard L. Klemke, Xinning Jiang, Sunkyu Choi, and Jonathan A. Kelber

 

13. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins

Molly Lowndes and W. James Nelson

 

14. Purification of Native Arp2/3 Complex from Bovine Thymus

Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick

 

15. Purification of Arp2/3 Complex from Saccharomyces Cerevisiae

Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick

 

16. Measurement and Analysis of in vitro Actin Polymerization

Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick

 

17. Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions

Simon Scrace, Eric O’Neill, Ester M. Hammond and Isabel M. Pires

 

18. Measuring Chemotaxis Using Direct Visualisation Microscope Chambers

Andrew J Muinonen-Martin, David A. Knecht, Douwe M. Veltman, Peter A. Thomason, Gabriela Kalna, and Robert H. Insall

 

19. In Vitro Microtubule Severing Assays

Natasza E. Ziółkowska and Antonina Roll-Mecak

 

20. VE-Cadherin Status as an Indicator of Microvascular Permeability

Elizabeth Monaghan-Benson and Keith Burridge

 

21. High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis

Ved P. Sharma, David Entenberg, and John Condeelis

 

22. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells

Jose Javier Bravo-Cordero, Yasmin Moshfegh, John Condeelis, and Louis Hodgson

 

23. Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion

Joshua S. McLane, Nicholas J. Schaub, Ryan J. Gilbert, and Lee A. Ligon

 

24. Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions

Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap

Blurb

Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist.