Adamts Proteases: Methods and Protocols

ISBN-13: 9781493997008 / Angielski / Miękka / 2020 / 299 str.

Apte, Suneel S.

Opis

This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and thorough, ADAMTS Proteases: Methods and Protocols is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature. 

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. ADAMTS Proteins: Concepts, Challenges, and Prospects

Suneel S. Apte

2. Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology

Carlos Peris-Torres, Orlando Serrano, María del Carmen Plaza-Calonge and Juan Carlos Rodríguez-Manzaneque

3. Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins using Cell-Based Assays

Steven John Berardinelli and Robert S. Haltiwanger

4. Challenges and Solutions for Purification of ADAMTS Proteases: An Overview 

Alain C. Colige

5. Purification of Native or Recombinant ADAMTS2 and Procollagen I Cleavage Assay

Alain C. Colige

6. ADAMTS7: Recombinant Protein Expression and Purification

Rens de Groot

7. Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants

Milan M. Fowkes and Ngee H. Lim

8. Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins

Takao Kohno, Himari Ogino, Yuko Yamakage, and Mitsuharu Hattori

9. Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain

Himari Ogino, Yuko Yamakage, Mihoshi B. Yamashita, Takao Kohno, and Mitsuharu Hattori

10. Chemical Modification of Proteoglycanases with Biotin

Salvatore Santamaria

11. Analysis of Aggrecanase Activity using Neoepitope Antibodies

Salvatore Santamaria and Kazuhiro Yamamoto

12. Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4

Mukti Singh, Stuart A. Cain, and Clair Baldock

13. Purification of Recombinant ADAMTSL2 

Mukti Singh and Clair Baldock

14. Expression Analysis by RNAscope™ In Situ Hybridization  

Timothy J. Mead and Suneel S. Apte

15. Analysis of ADAMTS Effects on Cell Adhesion and Migration

Jordi Lambert and Dylan R. Edwards

16. Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils

Dirk Hubmacher

17. Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton 

Timothy J. Mead 

18. Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins

Valentin Nelea and Dieter P. Reinhardt

19. Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins Rongmo Zhang, Heena Kumra, and Dieter P. Reinhardt

20. Visualization of Perineuronal Nets in Central Nervous System Tissue Sections

Luke Souter and Jessica C. F. Kwok

 21. Visualization and Quantification of Pericellular Matrix

Timothy J. Mead and Suneel S. Apte

22. Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP Activity

Chun-Yao Yang, Linda Troeberg, and Simone D. Scilabra

23. Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates

Rahel Schnellmann and Ruth Chiquet-Ehrismann

24. Mapping the N-Terminome in Tissue Biopsies by PCT-TAILS

Louise Bundgaard, Simonas Savickas, and Ulrich auf dem Keller