A pneumococcal protein glycocapture approach

ISBN-13: 9786202101806 / Hiszpański / Miękka / 2018 / 68 str.

Alfonso Olaya Abril

Opis

Emergence of pneumococcal multi-drug resistant clinical isolates prompts the need for a more effective vaccine for the prevention of disease. For this purpose, glycoproteins could be excellent candidates because of their implication in a number of biological processes, such as cell-to-cell recognition and the host immune response. In this study, methods widely used to capture potential surface proteins independently of their glycosylation status were carried out, and Concanavalin A (ConA) and Hydrazide subproteomes were obtained from different pneumococcal subcellular fractions (membrane, lysate, secretome and membrane-derived vesicles (MVs)) followed by protein LC-MS/MS analysis. In addition, a 2DE multilectinblotting (2DEMLB) analysis, using ConA, WGA, UEA, BC-1 and PNA lectins, was performed. Alfonso Olaya Abril , Lidia Gómez Gascón, Irene Jiménez Munguía, Michael J. McConnell, Meritxell García Quintanilla, Ignacio Obando Santaella, Manuel José Rodríguez Ortega.

O autorze

Graduate in Biochemistry and Biology, Post-graduated as Master in Molecular, Cellular and Genetics Biotechnology and Doctor in Biomedicine from University of Córdoba (UCO). Extraordinary Prize of Degree (Biochemistry), Extraordinary Doctorate Award and Gonzalo Miño Fugarolas Research Award.