Mass Spectrometric Analysis of Oligonucleotide and DNA Adducts : Computational, Enzymatic and Mass Spectrometric Methods for DNA Adducts Analysis in Cancer Related Research - The Past, Present and

ISBN-13: 9783639146851 / Angielski / Miękka / 2009 / 160 str.

Qing Liao

Opis

The assignment of the sites of modification by different carcinogens within a target sequence is a very important topic in cancer etiology and a critical part in the analysis of DNA adducts is to determine the site and frequency of modification on the DNA strands in order to better understand the relationship between the chemical behavior of different carcinogens and mutation hot spots. The initial focus was on the analysis of DNA adducts as monomeric units , i.e., nucleosides or nucleotides. In order to correlate the adducts formation and gene sequence, it is necessary to have a fast and accurate methodology for structural characterization of oligonucleotide-carcinogen adducts from in vitro and in vivo sources using on-line separation coupled with MS or tandem MS. The aim of this book s research has been to develop HPLC-MS methodology for the analysis of DNA adducts in the form of oligonucleotides. To achieve this goal, a thorough literature review was conducted and a systematic approach with carefully designed experiments was carried out along with a discussion on presents the future prospects of the research.

O autorze

Dr. Qing Liao obtained her B.S. and M.S. in analytical
chemistry from the University of Mississippi. She earned her
Ph.D. in mass spectrometry from Northeastern University. Dr.
Liao worked for Pharmacopeia and Pfizer before became the
manager of the Mass Spectrometry Facility of Harvard University
and remained there for seven years.