Protein Expression in Mammalian Cells: Methods and Protocols

ISBN-13: 9781617793516 / Angielski / Twarda / 2011 / 276 str.

James L. Hartley

Opis

Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.

Recenzje

From the reviews:

"This book is really a great help for all those that wish to enter the field and I am sure even for those who are already involved with recombinant protein production in mammalian cells. ... the book is intended as well for basic researchers aimed to study native or recombinant proteins to highlight ... protein misfolding or the role of proteins in cancer and in general is intended for all those scientists aiming to contribute to solve the many mysteries still connoting the protein world." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)

Spis treści

1. Why Proteins in Mammalian Cells?

            James L. Hartley

 

2. Large Scale Transfection of Mammalian Cells

            Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm

 

3. Selection of High Expressing Mammalian Cells by Surface Display of Reporters

            Christine T. DeMaria

 

4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles

            Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash

 

5. Transfection of Difficult-to-Transfect Primary Mammalian Cells

            Oliver Gresch and Ludger Altrogge

 

6. Stable Protein Expression in Mammalian Cells Using Baculoviruses

            Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch

 

7. Using Matrix Attachment Regions to Improve Recombinant Protein Production

            Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod

 

8. Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells

            Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald

 

9. Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells

            Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang

 

10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells

            Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens

 

11. Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker

            Say Kong Ng

 

12. Tools for Co-Producing Multiple Proteins in Mammalian Cells

            Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia

 

13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases

            Paula Magnelli, Alicia Bielik, and Ellen Guthrie

 

14. Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA

            Renate Kunert and Karola Vorauer-Uhl

 

15. Methods for Constructing Clones for Protein Expression in Mammalian Cells

            Takefumi Sone and Fumio Imamoto

 

16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells

            Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito