Nitric Oxide: Methods and Protocols

ISBN-13: 9781617379635 / Angielski / Twarda / 2010 / 226 str.

Helen O. McCarthy; Jonathan A. Coulter

Opis

The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.

Spis treści

1. Nitric Oxide Physiology and Pathology David G. Hirst and Tracy Robson Part I: Detection and Quantification of Nitric Oxide 2. Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide Kai Rothkamm and Susanne Burdak-Rothkamm 3. Determination of S-Nitrosothiols in Biological Fluids by Chemiluminescence Enika Nagababu and Joseph M. Rifkind 4. Measurement of Nitrite in Blood Samples Using the Ferricyanide-Based Hemoglobin Oxidation Assay Barbora Piknova and Alan N. Schechter 5. Selective Fluorescent Activation for Bioimaging the Expression of Nitric Oxide in Cellular and In vivo Systems Junfeng Zhang and Hao Hong 6. Real Time Measurement of Murine Hippocampus NO Levels in Response to Cerebral Ischemia/Reperfusion Xiaoxiang Zheng, Kezhou Liu, and Yong Yang 7. Detection of Low Levels of Nitric Oxide Using an Electrochemical Sensor Yong Chool Boo, Gyeong In Mun, Sarah L. Tressel, and Hanjoong Jo 8. Determination of the Scavenging Capacity Against Reactive Nitrogen Species by Automatic Flow Injection-Based Methodologies Marcela A. Segundo, Luís M. Magalhães, Joana P.N. Ribeiro, Marlene Lúcio, and Salette Reis 9. Aqueous Measurement of Nitric Oxide Using Membrane Inlet Mass Spectrometry David N. Silverman and Chingkuang Tu 10. Quantum Cascade Laser Technology for the Ultrasensitive Detection of Low Level Nitric Oxide Angela Elia, Pietro Mario Lugarà, Cinzia Di Franco, and Vincenzo Spagnolo 11. Determination of In vivo Nitric Oxide Levels in Animal Tissues Using a Novel Spin Trapping Technology Anatoly F. Vanin and Alexander A. Timoshin Part II: Nitric Oxide Generation 12. Beta Cell Protection by Inhibition of iNOS through Lentiviral Vector-Based Strategies Sean O. Hynes, Cillian McCabe, and Timothy O’Brien 13. Characterization of Nitric Oxide Delivery Systems Produced By Various Nanotechnologies Chi H. Lee 14. Nitric Oxide Releasing Nanoparticle Synthesis and Characterization George Han, Adam J. Friedman, and Joel M. Friedman 15. NOS Antagonism Using Viral Vectors as an Experimental Strategy: Implications for In vivo Studies of Cardio-Vascular Control and Peripheral Neuropathies Beihui H. Liu, James Hewinson, Haibo Xu, Francisco Montero, Carmen R. Sunico, Federico Portillo, Julian F.R. Paton, Bernardo Moreno-López, and Sergey Kasparov