Neuropeptide Techniques

ISBN-13: 9781617375873 / Angielski / Miękka / 2010 / 160 str.

Illana Gozes

Opis

Neuropeptides exhibit multiple functions as hormones and regulators. As such, many techniques are required to understand the contribution and functions of neuropeptides in health and disease processes. Research in the neuropeptide field is aimed toward a better understanding of the physiology of the human body and toward the development of better diagnostics and therapeutics. This book will familiarize the audience with forefront techniques in the field.

Recenzje

From the reviews:

"Novel molecular techniques have been developed to better understand peptide function in health and disease. This book deals with the most recent protocols for investigation of peptide function. ... Neuroscientists are the main audience. ... With well written text and excellent diagrams, the book delves into the intricacies of peptide research, including tissue preparation, methods of DNA inoculation, and experimental mouse models of peptide research. ... is a good book for neuroscience libraries." (Celso Agner, Doody's Review Service, June, 2008)

Spis treści

David Dangoor, Sara Rubinraut, Mati Fridkin and Illana Gozes The Design, Synthesis and Biological Evaluation of Peptide Analogs: VIP as an Example Illana Gozes, Miri Holtser-Cochav and Karin Vered Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example Inna Divinski, Inbar Pilzer and Illana Gozes Primary Cell Cultures and Cell Lines Inbar Pilzer, Inna Divinski and Illana Gozes Transfection of DNA into Cells Holter J.L., Davies J.S.,Man P-S., Wells T.and Carter D.A. Transgenic Delivery and Detection of GFP in Neuropeptide Neurons Sergey E. Ilyin and Carlos R. Plata-Salamán Integrative Technologies for Neuropeptide Characterization Niamh X. Cawley, Tulin Yanik , Irina Arnaoutova, Hong Lou, Nimesh Patel, and Y. Peng Loh Techniques in Neuropeptide Processing, Trafficking and Secretion Weihong Pan and Abba J. Kastin In Vivo Techniques Quantifying Blood-Brain Barrier Permeability to Small Proteins in Mice Terry W. Moody, Michael Schumann and Robert T. Jensen Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs Joanna M. Hill, Maria A. Lim, and Madeleine M. Stone Developmental Milestones in the Newborn Mouse

O autorze

Illana Gozes is a Professor of Clinical Biochemistry, the Lily and Avraham Gildor Chair for the Investigation of Growth Factors, Head of the Dr. Diana and Zelman Elton Elbaum Laboratory for Molecular Neuroendocrinology, and a member of the Sackler Faculty of Medicince at Tel Aviv University in Israel. In addition, she is the Editor in Chief of Humana Press's groundbreaking Journal of Molecular Neuroscience.

Blurb

Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Methods covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book explains primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Methods provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research.