Hormone Assays in Biological Fluids

ISBN-13: 9781617372995 / Angielski / Miękka / 2010 / 258 str.

Michael J. Wheeler; William D. Fraser; J. S. Morley Hutchinson

Opis

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Recenzje

From the reviews:

"Interest in hormone diagnostics has developed dramatically over the last decades ... . In summary, this book is written by knowledgeable experts in the field of hormone analysis in biological fluids ... . It provides a valuable collection of assays, described in a practical way, so they can be rapidly implemented by laboratorians looking for hormone assays suitable for research purposes or as alternatives to commercial immunoassays." (Linda M. Thienpont, Clinical Chemistry, Vol. 52 (9), 2006)

 

"...a wonderful resource for all thoe who, at some point, face decisions about the most suitable hormone assay for a particular situation." -TRENDS in Endocrinology and Metabolism

Spis treści

Immunoassay Techniques Michael J. Wheeler High-Performance Liquid Chromatography for Hormone Assay John W. Honour Gas Chromatography-Mass Spectrometry John W. Honour An Introduction to PCR Techniques Gill Rumsby Methods for the Investigation of Thyroid Function Christine R. Squire Assays for LH, FSH, and Prolactin Michael J. Wheeler Adrenocorticotropic Hormone and Related Peptides Les A. Perry Measurement of Glucocorticoids in Biological Fluids Geoff Holder Urinary Steroid Profiling Norman F. Taylor Measurement of Aldosterone in Blood Marion L. Cawood Measurement of Plasma Renin Activity Marion L. Cawood Measurement of Androgens Michael J. Wheeler Measurement of Gut Hormones in Plasma Shahrad Taheri, Mohammad Ghatei, and Steve Bloom Measurement of Melatonin and 6-Sulphatoxymelatonin Benita Middleton Index

Blurb

Although automated systems can measure all common hormones in human blood, they are expensive and often incapable of measuring hormones in urine, saliva, and animals. In Hormone Assays in Biological Fluids, expert researchers who have developed and applied significant new assays describe in step-by-step detail readily reproducible methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can develop novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and polymerase chain reaction techniques help readers choose the best methods for their purpose. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, Hormone Assays in Biological Fluids illuminates the many different methods available for a broad range of hormone assays, enabling researchers to choose the optimal existing methods or develop novel assays for their specialized purposes.