osteoporosis: methods and protocols

ISBN-13: 9781588298287 / Angielski / Twarda / 2008 / 354 str.

Westendorf, Jennifer

Opis

In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis."

Spis treści

Table of Contents (* indicates corresponding author) In vitro models 1. Isolation and Culture of Rodent Osteoprogenitor Cells Thomas A. Owen* and Lydia C. Pan 2. Osteoclast Culture and Resorption Assays Elizabeth W. Bradley and Merry Jo Oursler* 3. Assessing New Bone Formation in Neonatal Calvarial Organ Cultures Khalid S. Mohammad, John M. Chirgwin and Theresa Guise* 4. Detection of Apoptosis of Bone Cells In Vitro Teresita Bellido*and Lilian I. Plotkin In vivo rodent models 5. In Vivo Parathyroid Hormone Treatments and mRNA Isolation and Analysis Xin Li, Ling Qin and Nicola C. Partridge* 6. Assessment of Bone Formation Capacity Using In Vivo Transplantation Assays: Procedure and Tissue Analysis Badem M. Abdalleh, Nicholas Ditzel and Moustapha Kassem* 7. Ossicle and Vossicle Implant Model Systems Glenda J. Pettway and Laurie K. McCauley* 8. Utility of the Ovariectomized (OVX) Rat as a Model for Human Osteoporosis In Drug Discovery Yogendra P. Kharode*, Michael C. Sharp and Peter V. N. Bodine 9. Orchidectomy (ORX) Models of Osteoporosis Stéphane Blouin, Hélène Libouban, Marie Françoise Moreau and Daniel Chappard* Methods of gene delivery 10. Gene Delivery by Adenoviruses Renny Franceschi* and Chunxi Ge 11. Lentiviral Delivery of shRNA Constructs into Osteoblasts Marc N. Wein, Dallas C. Jones and Laurie H. Glimcher* 12. Gene Delivery by Retroviruses Valerie Deregowski and Ernesto Canalis* Molecular and GeneticTechniques 13. Chromatin Immunoprecipitation (ChIP) Assays: Application of ChIP-on-chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells Margaret van der Deen, Mohammad Q. Hassan, Jitesh Pratap, Nadiya M. Teplyuk, Daniel W. Young, Amjad Javed, Sayyed K. Zaidi, Jane B. Lian, Martin Montecino, Janet L. Stein, Gary S. Stein and Andre J. van Wijnen* 14. Identification of Transcription Factor Target Genes by ChIP Display (CD) Artem Barski, Steve Pregizer and Baruch Frenkel* 15. Application of Laser Capture Microdissection (LCM) Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo Dafna Benayahu*, Rina Socher, and Irena Shur 16. Quantitative Trait Loci (QTL) Mapping Dong-Hai Xiong, Jian-Feng Liu, Yan-Fang Guo, Yan Guo, Tie-Lin Yang, Shu-Gui Chen, Hui Jiang, Yuan Chen, Fang Yang, Robert R. Recker and Hong-Wen Deng* Imaging Techniques 17. In Situ Nuclear Organization of Regulator Machinery Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein* 18. Bioluminescent Imaging in Bone Yoram Zilberman, Yossi Gafni, Gadi Pelled, Zulma Gazit, and Dan Gazit* 19. Micro Computed Tomography – A Method for the Nondestructive Evaluation of the Three-Dimensional Structure of Biological Specimens Martin Stauber and Ralph Müller* 20. Fourier Transform Infrared (FT-IR) Microspectroscopy and Microscopic Imaging Samuel Gourion-Arsiquaud, Paul A. West and Adele L. Boskey* Mechanical