Serpins: Methods and Protocols

ISBN-13: 9781493993529 / Angielski / Miękka / 2019 / 276 str.

Alexandra Lucas

Spis treści

Dedication…

Preface…

Table of Contents…

Contributing Authors…

1) Overview of Serpins and Their Role in Biological Systems

Alexandra Lucas, Jordan Yaron, Liqiang Zhang, and Sriram Ambadapadi

2) Methods for Determining and Understanding Serpin Structure and Function: X-Ray Crystallography 

Brian P. Mahon, and Robert McKenna

3) Serpin Phage Display: Use of a T7 System to Probe Reactive Centre Loop Libraries with Different Serine Proteinases 

Lucas R. de Souza, Benjamin M. Scott, Varsha Bhakta, David A. Donkor, Darian L. Perruzza, and William P. Sheffield

4) Kinetic Measurement of Serpin Inhibitory Activity by Real-Time Fluorogenic Biochemical Assay

Jordan R. Yaron, Sriram Ambadapadi, Liqiang Zhang, and Alexandra Lucas

5) Methods for Identifying Virus-Derived Serpins

Masmudur M. Rahman

6) In Vitro Approaches for the Assessment of Serpin Polymerization

Emma L. K. Elliston, David A. Lomas, and James A. Irving

7) Cellular Models for the Serpinopathies

Annamaria Fra, Emanuela D’Acunto, Mattia Laffranchi, and Elena Miranda

8) Binding of Serpins to Immobilized Phospholipids and Phospholipids in Suspension

Felix C. Wahlmüller, Judit Mihaly-Bison, and Margarethe Geiger

9) Viral Serpin Reactive Center Loop (RCL) Peptides: Design and Testing

Liqiang Zhang, Jordan R. Yaron, Sriram Ambadapadi, and Alexandra Lucas

10) In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models

Sihong Song, Yuanqing Lu, and Ahmed S. Elshikha

11) Analysis of In Vivo Serpin Functions in Models of Inflammatory Vascular Disease 

Hao Chen, Sriram Ambadapadi, Erbin Dai, Liying Liu, Jordan Yaron, Liqiang Zhang, and Alexandra Lucas

12) In Vivo Analysis of Alpha 1 Antitrypsin Functions in Autoimmune Disease Models

Sihong Song, Yuanqing Lu, and Ahmed S. Elshikha

13) Serpins in Venous Thrombosis and Venous Thrombus Resolution

Subhradip Mukhopadhyay, Tierra A. Johnson, Rajabrata Sarkar, and Toni M. Antalis

14) Next Generation Sequencing Library Preparation for 16S rRNA Microbiome Analysis after Serping Treatment

Juan Ortis-Maldonado, Jordan R. Yaron, Liqiang Zhang, and Alexandra R. Lucas

15) Methods for Assessing Serpins as Neuroprotective Therapeutics

Jacek M. Kwiecien

16) Serpins: Development as Clinical Therapeutics

Alexandra Lucas, Jordan Yaron, Liqiang Zhang, and Grant McFadden

17) Appendix 1

Serine Protease Assays: Measuring the Enzyme Targets for Serpins, Serine Protease Inhibitors

Jovil Kannampuzha, Anatharam Kalya, and Alexandra Lucas

Blurb

This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models. The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Serpins: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this evolving field.