Pulse Field Gel Electrophoresis: Methods and Protocols

ISBN-13: 9781493955886 / Angielski / Miękka / 2016 / 278 str.

Kieran Jordan; Marion Dalmasso

Opis

This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

 Part I: Introduction to PFGE

 1. Pulsed-field Gel Electrophoresis for Disease Monitoring and Control

John Besser

 

2. Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network

Benjamin Félix, Sophie Roussel, and Bruno Pot

 

3. PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies

Marion Dalmasso and Kieran Jordan

 

4. Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak

Niall De Lappe and Martin Cormican

 

 Part II: PFGE and Pathogenic Bacteria

 5. Pulsed-Field Gel Electrophoresis for Listeria monocytogenes

Laura Luque-Sastre, Séamus Fanning,  and Edward M. Fox

 

6. Pulsed-field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species

Qiongqiong Yan and Séamus Fanning

 

7. Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains

Paul Drean and Edward M. Fox

 

8. Pulsed-Field Gel Electrophoresis of Staphylococcus aureus

George R. Golding, Jennifer Campbell, Dave Spreitzer, and Linda Chui

 

9. The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile

Wondwossen A. Gebreyes and Pamela R. Fry

 

10. Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis

Carolina Lúquez, Lavin A. Joseph, and Susan E. Maslanka

 

11. Pulsed Field Gel Electrophoresis of Yersinia pestis

Tamara Revazishvili and Judith A. Johnson

 

12. Pulsed Field Gel Electrophoresis of Group A streptococci

Luca Agostino Vitali, Giovanni Gherardi, and Dezemona Petrelli

 

13. Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni

Ping Zhou and Omar A. Oyarzabal

 

14. Pulsed-field Gel Electrophoresis of Pseudomonas aeruginosa

Honghua Hu and Jim Manos

 

15. PFGE for Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) and non-O157 STEC

Patricia Jaros, Muriel Dufour, Brent Gilpin, Molly M. Freeman, and Efrain M. Ribot

 

16. Pulsed Field Gel Electrophoresis (PFGE) of Salmonella enterica

Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, and Nicola Pugliese

 

  

PART III: PFGE and Non-Pathogenic Bacteria

 17. PFGE Protocols to Distinguish Subspecies of Lactococcus lactis

Pascal Le Bourgeois, Delphine Passerini, Michèle Coddeville, Maéva Guellerin, Marie-Line Daveran-Mingot, and Paul Ritzenthaler

 

18. The use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus genus

Tomasz Gosiewski and Monika Brzychczy-Wloch

 

19. Pulsed Field Gel Electrophoresis for Leuconostoc mesenteroides and pseudomesenteroides

Victoria Chuat and Marion Dalmasso

 

20. Pulsed Field Gel Electrophoresis for Bifidobacterium

Esther Jiménez, Marta Gómez, and Laura Moles

 

21. Pulsed Field Gel Electrophoresis for Dairy Propionibacteria

Victoria Chuat, Rosangela de Freitas, and Marion Dalmasso