Mass Spectrometry in Metabolomics: Methods and Protocols

ISBN-13: 9781493912575 / Angielski / Twarda / 2014 / 360 str.

Daniel Raftery

Opis

Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids, cells or tissue, plants, foods and related samples and this information provides enormous detail on biological systems and their current status. Mass Spectrometry in Metabolomics: Methods and Protocols presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics, this volume includes a wide variety such as triple quads, time of flight, Fourier transform ion cyclotron resonance and even simple quadrupole systems. A wide range of studies are described, with samples ranging from blood and urine to tissue and even plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Mass Spectrometry in Metabolomics: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics.

Spis treści

Part I. Historical and Methodology Overview

 

1. Overview: of Mass Spectrometry Based Metabolomics: Opportunities and Challenges

            G. A. Nagana Gowda and Danijel Djukovic

 

Part II. Global LC-MS Based Metabolic Profiling

 

2. Global Metabolic Profiling using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry

            Yunpeng Qi, Yunlong Song, Haiwei Gu, Guorong Fan, and Yifeng Chai

3. LC-MS Profiling to Link Metabolic and Phenotypic Diversity in Plant Mapping Populations

Camilla B. Hill, Antony Bacic, and Ute Roessner

 

4. Mitochondrial Metabolomics Using High-Resolution Fourier-Transform Mass Spectrometry

            Young-Mi Go, Karan Uppal, Douglas I. Walker, ViLinh Tran, Lauriane Dury, Frederick H. Strobel, Hélène Baubichon-Cortay, Kurt D. Pennell, James R. Roede, and Dean P. Jones

 

5. Sample Preparation Methods for LC-MS Based Global Aqueous Metabolite Profiling

            Antoni Beltran, Sara Samino, and Oscar Yanes

 

Part III. Global GC-MS and CE-MS Based Metabolic Profiling

 

6. Methods of Discovery-Based and Targeted Metabolite Analysis by Comprehensive Two-Dimensional Gas Chromatography with Time-of-Flight Mass Spectrometry Detection

            Luke C. Marney, Dr. Jamin C. Hoggard, Kristen J. Skogerboe, and Robert E. Synovec

 

7. Analysis of Mouse Liver Metabolites by GC×GC–TOF MS

            Xue Shi, Xinmin Yin, and Xiang Zhang

 

8. Metabolite Fingerprinting by Capillary Electrophoresis-Mass Spectrometry

            Antonia Garcia, Shama Naz, and Coral Barbas

 

Part IV. Isotope Labeling Methods

 

9. Quantitative Metabolomic Profiling using Dansylation Isotope Labeling and Liquid Chromatography Mass Spectrometry

Ruokun Zhou and Liang Li

 

10. Quantitative Analysis of Amino and Organic Acids by Methyl Chloroformate Derivatization and GC-MS/MS Analysis

Hans Fredrik Nyvold Kvitvang, Kåre A. Kristiansen, Stina K. Lien, and Per Bruheim

 

11. Stable Isotope Labeled Tracers for Metabolic Pathway Elucidation by GC-MS and FT-MS

Richard M. Higashi, Teresa W-M. Fan, Pawel K. Lorkiewicz, Hunter N.B. Moseley, and Andrew N. Lane

 

Part V. Targeted Aqueous Metabolite Profiling

 

12. Multiplexed, Quantitative and Targeted Metabolite Profiling by LC-MS/MRM

Ru Wei, Guodong Li, and Albert B. Seymour

 

Part VI. Lipid Profiling Methods

 

13. Multi-Dimensional Mass Spectrometry-Based Shotgun Lipidomics

            Miao Wang and Xianlin Han

 

14. Comprehensive Quantitative Determination of PUFA-Related Bioactive Lipids for Functional Lipidomics using High Resolution Mass Spectrometry

Mojgan Masoodi and Dietrich A. Volmer

 

15. Ultra-Performance Liquid Chromatography Mass Spectrometry Targeted Profiling of Bile Acids: Application to Serum, Liver Tissue and Cultured Cells of Different Species

            Juan Carlos García-Cañaveras, María Teresa Donato, and Agustín Lahoz

 

Part VII. Profiling Volatile Organic Compounds

 

16. Analysis of Volatile Organic Compounds in Exhaled Breath by Gas Chromatography-Mass Spectrometry combined with Chemometric Analysis

            Jan W. Dallinga, Agnieszka Smolinska, and Frederik-Jan van Schooten

 

17. Headspace SPME-GC-MS Metabolomics Analysis of Urinary Volatile Organic Compounds (VOCs)

            Shucha Zhang and Daniel Raftery

 

Part VIII. Profiling and Imaging using Atmospheric Sampling Methods

 

18. Metabolite Profiling by Direct Analysis in Real Time Mass Spectrometry

            Christina M. Jones, María Eugenia Monge, and Facundo M. Fernández

 

19. Analysis of Dried Blood Spots using DESI Mass Spectrometry

            Justin M. Wiseman and Joseph H. Kennedy

 

20. DESI-MS Imaging of Lipids and Metabolites from Biological Samples

            Livia S. Eberlin

 

21. Metabolic Imaging using Nanostructure-Initiator Mass Spectrometry (NIMS)

            Katherine B. Louie and Trent R. Northen

 

Part IX. Statistical Analysis Methods

 

22. Statistical Analysis and Modeling of Mass Spectrometry-Based Metabolomics Data

            Bowei Xi, Haiwei Gu, Hamid Baniasadi, and Daniel Raftery