Metabolic Pathway Engineering

ISBN-13: 9781071601945 / Angielski / Twarda / 2020 / 236 str.

Michael E. Himmel; Yannick J. Bomble

Spis treści

1. Overview and Future Directions

            Yannick J. Bomble and Michael E. Himmel

 

2. Genetics of Unstudied Thermophiles for Industry

            Daehwan Chung, Nicholas S. Sarai, Michael E. Himmel, and Yannick J. Bomble

 

3. Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum

            Shuen Hon, Liang Tian, Tianyong Zheng, Jingxuan Cui, Lee R. Lynd, and Daniel G. Olson

 

4. Methods for Metabolic Engineering of a Filamentous Trichoderma reesei

            Yat-Chen Chou, Arjun Singh, Qi Xu, Michael E. Himmel, and Min Zhang

 

5. Methods for Algal Protein Isolation and Proteome Analysis

            Eric P. Knoshaug, Alida T. Gerritsen, Calvin A. Henard, and Michael T. Guarnieri

 

6. An Improved Leaf Protoplast System for Highly Efficient Transient Expression in Switchgrass (Panicum virgatum L.)

            Chien-Yuan Lin, Hui Wei, Bryon S. Donohoe, Chandra L. Tucker, Melvin P. Tucker, and Michael E. Himmel

 

7. Characterizing Intracellular Proteomes for Microbes: An Experimental Approach Using Label-Free Protein Quantitation

            Paul E. Abraham and Robert L. Hettich

 

8. Bacterial Differential Expression Analysis Methods

            Sagar Utturkar, Asela Dassanayake, Shilpa Nagaraju, and Steven D. Brown

 

9. Measuring Biomass-Derived Products in Biological Conversion and Metabolic Process

            Chang Geun Yoo, Yunqiao Pu, and Arthur J. Ragauskas

 

10. Crystallography of Metabolic Enzymes

            Markus Alahuhta, Michael E. Himmel, and Vladimir Lunin

 

11. Measuring Metabolic Enzyme Performance

            Amanda Williams-Rhaesa and Michael W.W. Adams

 

12. Gene Editing Technologies for Biofuel Production in Thermophilic Microbes

            Sharon Smolinski, Emily Freed, and Carrie Eckert

 

13. Software and Methods for Computational Flux Balance Analysis

            Peter C. St. John and Yannick J. Bomble

 

14. Dynamic Flux Analysis: An Experimental Approach of Fluxomics

            Wei Xiong, Huaiguang Jiang, and PinChing Maness

 

15. Network Modeling of Complex Data Sets

            Piet Jones, Deborah Weighill, Manesh Shah, Sharlee Climer, Jeremy Schmutz, Avinash Sreedasyam, Gerald Tuskan, and Daniel Jacobson

 

16. Connecting Microbial Genotype with Phenotype in the Omics Era

            Yongfu Yang, Mengyu Qiu, Qing Yang, Yu Wang, Hui Wei, and Shihui Yang

Blurb

This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost. 

Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes.