Celiac Disease: Methods and Protocols

ISBN-13: 9780896036505 / Angielski / Twarda / 2000 / 288 str.

Michael N. Marsh

Opis

Research into gluten sensitivity has never been more popular nor more exciting. Thus a call for a new book, Celiac Disease: Methods and Protocols, devoted entirely to techniques and technology seemed a most appropriate undertaking. I am therefore grateful to Professor J. M. Walker for inviting me to complete this task for Humana Press. To do this would have been imp- sible without the contributions of friends and colleagues from around the world who have devoted so much interest to the project. It has also been necessary for them to master the unique chapter-writing skills required of every ma- script published in this series of laboratory monographs. With regard to gluten sensitivity we are in a period of great change, occasioned by the introduction of reproducible methods for cloning lymp- cytes, the application of physical methods to identify gluten sequences as T-cell antigens, the study of peptide responses in vitro and in vivo by either jejunal or rectal challenge, elucidating the locations of other genes concerned in pathogenesis, or the use of elegant immunohistocytochemical and mRNA probing techniques for analyzing the finer points of the mucosal inflam- tory response to gluten.

Recenzje

"Of interest to gastroenterologists, pathologists and those working in research on celiac disease, a disease linked by a so far unknown mechanism to type 1 diabetes." -Journal of Pediatric Endocrinology & Metabolism

Spis treści

Celiac Disease: A Brief Overview, Debbie Williamson and Michael N. Marsh. Genotyping Methodologies, Stephen Bevan and Richard S. Houlston. From Linkage to Genes: Positional Cloning, Stephen Bevan and Richard S. Houlston. Linkage and the Transmission Disequilibrium Test in Complex Traits: Celiac Disease as a Case Study, Stephen Bevan and Richard S. Houlston. Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats, Arthur S. Tatham, Simon M. Gilbert, Roger J. Fido, and Peter R. Shewry. Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry, Peter A. van Veelen, Jan W. Drijfhout, and Frits Koning. Synthetic Peptide Libraries for T-Cell Epitope Identification, Hoebert S. Hiemstra, Jan W. Drijfhout, and Frits Koning. Characterization of HLA-DQ-Specific Peptide-Binding Motifs, Yvonne van de Wal, Reinout Amons, and Frits Koning. Studies of Gliadin-Specific T Cells in Celiac Disease, Øyvind Molberg, Stephen N. McAdam, Knut E. A. Lundin, and Ludvig M. Sollid. Morphometric Analysis of Intestinal Mucosa: The Measurement of Volume Compartments and Cell Volumes in Human Intestinal Mucosa, Michael N. Marsh, Peter T. Crowe, Kieran J. Moriarty, and Arzu Ensari. Morphometry of Rectal Mucosa, Arzu Ensari, Michael N. Marsh, Peter T. Crowe, and Kieran J. Moriarty. Organ Culture of Rectal Mucosa: In Vitro Challenge with Gluten in Celiac Disease, Guiseppe Mazzarella, Franco Paparo, Maria Maglio, and Riccardo Troncone. In Situ Hybridization, Conrad G. Beckett and Paul Ciclitira. Quantification of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease, Ellen M. Nilsen, Finn-Eirik Johansen, and Per Brandtzaeg. Immunohistochemistry in Research and Diagnosis of Celiac Disease, Per Brandtzaeg and Frode L. Jahnsen. Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease, Walburga Dieterich, Ernst Otto Riecken, and Detlef Schuppan.Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease, David J. Unsworth. Whole Gut Lavage Fluid Analysis: A Minimally Invasive Method for Study of Mucosal Immunity and Inflammation, Subrata Ghosh, Anna Dahele, Hazel E. Drummond, Syed S. Hoque, Kenneth Humphreys, and Ian D. R. Arnott. Index.

Blurb

With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.
Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.