Helicobacter Pylori Protocols

ISBN-13: 9780896033818 / Angielski / Twarda / 1997 / 274 str.

Christopher L. Clayton; Harry L. T. Mobley

Opis

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.

Recenzje

"The book was designed to provide up-to-date detailed protocols for the experienced either working
in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine

Spis treści

Introduction: Medical Significance of H. pylori Martin J. Blaser Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture Stewart Goodwin Serology and Urea Breath Test in the Diagnosis of H. pylori Infection Peter R. Hawtin The Detection of H. pylori by the Polymerase Chain Reaction Nicholas P. Mapstone PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples Stephen B. Fritz and T. Ulf Westblom In Vitro Susceptibility Testing of H. pylori, Alistair McLaren. Defined Media for H. pylori David J. Reynolds Studies of Respiration in H. pylori Roger J. Miles and Huai-Tzu Chang Use of NMR to Study H. pylori Metabolism Peter A. Chalk, Andrew D. Roberts, Alistair A. Davison, David J. Kelly, and Peter J. White Isolation of H. pylori Genomic DNA and Restriction Analysis Robert J. Owen and Jane Bickley Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori Wee Tee Differentiation of H. pylori Strains Using PCR RFLP Harry L. T. Mobley and Paul A. Foxall PCR-DNA Sequence Typing Agnès Labigne H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method Douglas E. Berg, Janaki Lelwala-Guruge, Engin T. Incecik, Kalpana Srivastava, and Natalia S. Akopyants Molecular Methods for Detecting Ulcerogenic Strains of H. pylori John C. Atherton H. pylori DNA Transformation by Natural Competence and Electroporation Zhongming Ge and Diane E. Taylor Random Mutagenesis of the H. pylori Genome Agnès Labigne Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE) Nicholas Chang, Qin Jiang, and Diane E. Taylor Molecular Characterization of H. pylori SurfaceAntigens Peter Doig, Paul W. O'Toole, and Trevor J. Trust Isolation and Characterization of Porins from H. pylori Robert E. W. Hancock and Maurice Exner Methods for the Identification of H. pylori Host Receptors Thomas Borén, Torkel Wadström, Staffan Normark, Jeffrey I. Gordon, and Per G. Falk Host Responses to H. pylori: Molecular Analysis of Cytokine Gene Expression Susan M. Farmery and Jean E. Crabtree Nonprimate Animal Models of H. pylori Infection Andrew A. McColm The Nonhuman Primate Model for H. pylori Infection Andre Dubois and Douglas E. Berg Index

Blurb

In Helicobacter pylori Protocols, leading experts bring together an outstanding collection state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for culturing H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed to solve the difficult technical problems created by the application of today's powerful bacterial genetic and molecular cloning techniques to H. pylori.

Helicobacter pylori Protocols contains a wide variety of stand-alone techniques that are unfailingly reproducible. The book is certain to become a standard experimental and clinical laboratory reference for all those engaged in H. pylori work today.