Chemical Approaches to the Synthesis of Peptides and Proteins

ISBN-13: 9780849391422 / Angielski / Twarda / 1997 / 304 str.

Paul Lloyd-Williams; Paul Loyd-Williams; Ernest Giralt

Opis

This reference work evaluates synthesis methods and considers methods that show promise for future applications. The text describes efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more.

Spis treści

IntroductionAmino Acids and Peptide and Protein StructureAmino Acid Activation and CouplingProtection Schemes for Peptide SynthesisThe Synthesis of PeptidesSolid-Phase Peptide SynthesisThe Solid SupportProtection SchemesAttachment of the First Amino Acid to the Solid SupportChain ElongationCleavage of the Peptide from the ResinExamples of Protein Synthesis by Linear SPPSPeptide Synthesis in SolutionStrategic ConsiderationsProtection SchemesChain ElongationFinal Removal of Protecting GroupsSpecial Approaches to the Synthesis of Peptides in SolutionExamples of Peptide Synthesis in SolutionConvergent Approaches to the Synthesis of Large Peptides and ProteinsConvergent Solid-Phase Peptide SynthesisThe Coupling of Protected Peptide Segments in SolutionCoupling of Minimally Protected Peptides in Aqueous SolutionChemical Ligation of Peptide SegmentsFormation of Disulfide BridgesPeptides with Disulfide BridgesProtection of Cysteine in Peptide SynthesisChemical Methods for the Formation of Disulfide BridgesRegioselective Disulfide Bridge FormationPeptide LibrariesMultiple Peptide Synthesis in ParallelPeptide Library TechniquesExamples of Peptide Library SynthesisiEach chapter contains References

O autorze

Paul Lloyd-Williams (Universitat de Barcelona, Barcelona, Spain), Fernando Albericio (Parc Cientific de Barcelona, Spain) and Ernest Giralt (Biomedical Research Institute, Barcelona, Spain)