Plant Chromosomes : Laboratory Methods

ISBN-13: 9780849389191 / Angielski / Miękka / 1996 / 288 str.

Kiichi Fukui; Shigeke Nakayama

Opis

Finally -- a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies.

Spis treści

Plant Chromosomes at Mitosis, K. FukuiOutline of Mitotic ChromosomesPreparation Method for the Small Chromosome: Enzymatic Maceration/Air Drying (EMA) MethodPreparation Method for the Large Chromosome: Squash MethodPreparation Method for Chromosomal Samples of Woody PlantsPreparation Method for Chromosome Samples of Suspension CellsPreparation Method for Chromosome Samples of Flowers and LeavesPermanent Preparation of Chromosome SamplesPlant Chromosomes at Meiosis, P.G. Goicoechea and R. GiraldezIntroductionMeiotic Synchrony and Meiotic GradientsProceduresPlant Chromosomes at Metabolic Phase, K. TaniguchiIntroductionMorphological Classification by Distribution of Condensed HeterochromatinVisualizing CondensationApplication of Chromosome Banding TechniquesPolytene Chromosomes, W. NaglIntroductionOccurrence and Structure of Polytene Chromosomes in PlantsPreparationStaining MethodsDNA and Gene AnalysisDigitation and Computer AnalysisFuture ChallengesFlow Cytometry and Chromosome Sorting, J.S. Heslop-Harrison and T. SchwarzacherIntroductionProceduresFuture ApplicationsChromosome Dissection and Direct Cloning, Y. Kamisugi and K. FukuiIntroductionEquipmentMicrodissection of Plant ChromosomesDirect CloningDirect-Labeling and In Situ HybridizationChromosome Banding Methods, B. Friebe, T.R. Endo, and B.S. GillIntroductionPrinciples of Chromosome BandingProceduresIn Situ Hybridization, Y. MukaiIntroductionAdvances in In Situ HybridizationProceduresSister Chromatid Exchange and Replication Banding, B. Friebe and F. CortésIntroductionSister Chromatid Differentiation in Second Division ChromosomesSister Chromatid Differentiation in Third Division ChromosomesReplication BandingProceduresReplication of Chromosomes, K. Taniguchi and I. FujishigeIntroductionPrinciples of Replication BandingProceduresApplications Chromosome Manipulation in Wheat, J.P. Gustafson and J.W. SnapeIntroductionGenome Balance and IntrogressionRandom Homologous Alien TransfersIrradiation Technologies and Selection StrategiesHomologous Transfers by the Control of RecombinationAlien Introgression and Gene ExpressionConclusionsElectron Microscopy and Plant Chromosomes, J.S. Heslop-HarrisonIntroduction Developments in Electron MicroscopyGeneral Precautions for Electron Microscopy The SpecimenProcedureDNA:DNA In Situ HybridizationQuantification and Reconstruction of Three Dimensional Images from Serial SectionsTransfer of Spread Chromosome Preparations from Slides to Electron MicroscopeDevelopments and ConclusionsAnalysis of Chromosome Information, K. Fukui and S. NakayamaHistorical Perspective of Imaging Plant ChromosomesConcept of Image AnalysesInstrumentation and SoftwareProcedureAppendix I: Buffer SolutionsAppendix II: Tissue Culture MediaIndex

O autorze

Kiichi Fukui, Shigeki Nakayama