Dyneins: Dynein Mechanics, Dysfunction, and Disease

ISBN-13: 9780128094709 / Angielski / Twarda / 2017 / 530 str.

Stephen M. King

Opis

Dyneins: Structure, Biology and Disease, Second Edition, offers a broad view of dyneins mechanics, dysfunction, and disease, providing an overview of dyneins from structure and function, to dysfunction and disease. Since the first edition, enormous strides have been taken in understanding dynein structure, its organization in the axoneme, single molecule motor mechanics, and the consequences of defects for human biology, disease, and development. To account for these enormous strides, the second edition is extensively revised. Additionally, the coverage has expanded from 24 to 42 chapters, and is now housed in two volumes. Much of the expanded coverage occurs in Volume 2 which focuses on dynein dysfunction and disease, such as the role of dynein and cancer. Volume 1 covers the history and evolution of dyneins, dyneins in ciliary biology, and cytoplasmic dynein biology, while Volume 2 covers the structure and mechanics of dynein motors and dynein dysfunction and disease.

• Presents a broad-based and up-to date view of dynein mechanics, dysfunction, and disease
• Contains approaches from genetics, molecular biology, biochemistry, and biophysics discussed
• Provides companion website with movies of dynamic cell behavior
• Includes extensive chapters written by leading, global experts

Spis treści

I. Structure and Mechanics of Dynein Motors 1. Electron microscopy of isolated dynein complexes and the power stroke mechanism 2. Mechanism and regulation of dynein motors 3. Structural analysis of dynein intermediate and light chains 4. Biochemical purification of axonemal and cytoplasmic dyneins 5. Single molecule dynein motor mechanics in vitro 6. Biophysical properties of dynein in vivo 7. Mechanics of bidirectional cargo transport 8. Chemical probes for dynein 9. Computational modeling of dynein activity and the generation of flagellar beating waveforms

II. Dynein Dysfunction and Disease 10. Impacts of virus-mediated manipulation of host dynein 11. Use of mouse genetics to probe cytoplasmic dynein function 12. Cytoplasmic dynein and its regulators in neocortical development and disease 13. Cytoplasmic dynein dysfunction and neurodegenerative disease 14. Dynein dysfunction as a cause of primary ciliary dyskinesia and other ciliopathies 15. Severe skeletal abnormalities caused by defects in retrograde intraflagellar transport dyneins 16. Ciliary dynein dysfunction as a consequence of chronic alcohol exposure 17. Dynein-based motility of pathogenic protozoa 18. Dynein axonemal light chain 4: involvement in congenital mirror movement disorder 19. Does dynein influence the non-Mendelian inheritance of chromosome 17 homologues in male mice?

O autorze

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.