Virus-Induced Gene Silencing in Plants: Methods and Protocols

ISBN-13: 9781071607534 / Angielski / Miękka / 2021 / 261 str.

Vincent Courdavault; Sébastien Besseau

Spis treści

1. Virus-induced Gene Silencing in Lilies using Cucumber Mosaic Virus Vectors

Keisuke Tasaki, Masumi Yamagishi, and Chikara Masuta

 

2. Virus-Induced Gene Silencing in Poaceae Using a Foxtail Mosaic Virus Vector

Ying-Wen Huang, Chao-Yuan Chang, and Yau-Heiu Hsu

 

3. Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.)

Magda Lisette Arce-Rodríguez and Neftalí Ochoa-Alejo

 

4. Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species

Jinping Zhao, Haolang Jiang, Guanyu Wang, Zonghua Wang, Jingao Dong, and Junqi Song

 

5. Virus Induced Gene Silencing (VIGS) in Cassava using Geminivirus Agroclones

Syed Shan-e-Ali Zaidi, Kumar Vasudevan, Ezequiel Matias Lentz, and Hervé Vanderschuren

 

6. Virus Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum)

Maxime Chantreau and Godfrey Neutelings

 

7. Virus-Induced Gene Silencing to Investigate Alkaloid Biosynthesis in Opium Poppy

Rongji Chen, Xue Chen, Jillian M. Hagel, and Peter J. Facchini

 

8. A Biolistic-mediated Virus-Induced Gene Silencing in Apocynaceae to Map Biosynthetic Pathways of Alkaloids

Pamela Lemos Cruz, María Isabel Restrepo, Thomas Dugé de Bernonville, Audrey Oudin, Thibault Munsch, Arnaud Lanoue, Sébastien Besseau, Lucia Atehortùa, Nathalie Giglioli-Guivarc’h, Nicolas Papon, Marc Clastre, Inês Carqueijeiro, and Vincent Courdavault

 

9. Virus Induced Gene Silencing in Nepeta

Lira Palmer and Sarah E. O’Connor

 

10. Virus Induced Gene Silencing in Sweet basil (Ocimum basilicum)

Rajesh Chandra Misra, Shubha Sharma, Anchal Garg and Sumit Ghosh

 

11. Virus-Induced Gene Silencing for Gene Function Studies in Withania somnifera, an Important Indian Medicinal Plant

Dikki Pedenla Bomzan, H.B. Shilpashree, Anjali P., Sarma Rajeev Kumar, and Dinesh A. Nagegowda

 

12. A Prunus necrotic ringspot virus (PNRSV)-based Viral Vector for Characterization of Gene Functions in Prunus Fruit Trees

Hongguang Cui, Yinzi Li, and Aiming Wang

 

13. Virus-Induced Gene Silencing in Olive Tree (Oleaceae)

Konstantinos Koudounas, Margarita Thomopoulou, Elisavet Angeli, Dikran Tsitsekian, Stamatis Rigas, and Polydefkis Hatzopoulos

 

14. ALSV-based Virus-Induced Gene Silencing in Apple Tree (Malus x domestica L.)

Carolina Werner Ribeiro, Thomas Dugé de Bernonville, Gaëlle Glévarec, Arnaud Lanoue, Audrey Oudin, Olivier Pichon, Benoit St-Pierre, Vincent Courdavault, and Sébastien Besseau

 

15. Virus-Induced Gene Silencing for Functional Analysis of Flower Traits in Petunia

Shaun R. Broderick, Laura J. Chapin, and Michelle L. Jones

 

16. Virus-induced Gene Silencing in Rose Flowers

Huijun Yan, Zhao Zhang, Jean-Louis Magnard, Benoît Boachon, Sylvie Baudino, and Kaixue Tang

 

17. Virus-induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seedcoat: Description of an Effective Procedure using the transparent testa 2 Gene as a Selectable Marker

Christophe Hano, Samantha Drouet, and Eric Lainé

 

18. Virus-Based microRNA Silencing in Plants

Jinping Zhao, Guanyu Wang, Haolang Jiang, Tingli Liu, Jingao Dong, Zonghua Wang, Baolong Zhang, and Junqi Song

Blurb

This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.