Urothelial Carcinoma: Methods and Protocols

ISBN-13: 9781071632901 / Angielski

Michèle J. Hoffmann; Nadine T. Gaisa; Roman Nawroth

Opis

This fully updated volume explores recently improved avenues to study urothelial carcinomas. Beginning with several novel chapters on molecular characterization and urothelial carcinogenesis, the book continues with sections on cellular and animal models, biomarkers, and approaches for targeted therapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Urothelial Carcinoma: Methods and Protocols, Second Edition serves as a valuable resource to further increase our knowledge on urothelial carcinoma and also to aid research on numerous other cancers.

Spis treści

Part I: Molecular Characterization

 

1. Scoring Systems for Immunohistochemistry in Urothelial Carcinoma

            Mark-Sebastian Bösherz, Iryna V. Samarska, and Nadine Therese Gaisa

 

2. A Panel-Based Method for the Reproduction of Distinct Molecular Subtype Classifications of Muscle-Invasive Urothelial Bladder Cancer

            Csilla Olah and Tibor Szarvas

 

3. Analysis of Mutational Signatures Using the mutSignatures R Library

            Damiano Fantini and Joshua J. Meeks

 

4. A Drug Repurposing Pipeline Based on Bladder Cancer Integrated Proteotranscriptomics Signatures

            Marika Mokou, Shaman Narayanasamy, Rafael Stroggilos, Irina-Afrodita Balaur, Antonia Vlahou, Harald Mischak, and Maria Frantzi

 

5. Characterization of Native COMPASS Complex in Urothelial Carcinoma Cells by Size Exclusion Chromatography

            Christoph Peter, Wolfgang A. Schulz, and Patcharawalai Whongsiri

 

Part II: Urothelial Carcinogenesis

 

6. Reconstructing Phylogenetic Relationship in Bladder Cancer: A Methodological Overview

            Lancelot Seillier and Martin Peifer

 

7. Using Sister Chromatid Exchange Assay to Detect Homologous Recombination Deficiency in Epigenetically Deregulated Urothelial Carcinoma Cells

            Theodoros Rampias and Apostolos Klinakis

 

8. Identification of STAG2 Mutant Bladder Cancers by Immunohistochemistry

            Youngrok Park, Alana Lelo, Brent Harris, Deborah L. Berry, Krysta Chaldekas, Jung-Sik Kim, and Todd Waldman

 

Part III: Cellular and Animal Models

 

9. Genome-Wide CRISPR Screening for the Identification of Therapy-Resistance-Associated Genes in Urothelial Carcinoma

            Klaus Mantwill and Roman Nawroth

 

10. Tissue Slice Culture and Analysis of Tumor-Associated Hyaluronan in Urothelial Carcinoma

            William Donelan, Paul L. Crispen, and Sergei Kusmartsev

 

Part IV: Biomarkers

 

11. NGS-Based Tumor-Informed Analysis of Circulating Tumor DNA

            Iver Nordentoft, Karin Birkenkamp-Demtröder, and Lars Dyrskjøt

 

12. Considering the Effects of Modern Point-of-Care Urine Biomarker Assays in Follow-Up of Patients with High-Risk Non-Muscle Invasive Bladder Cancer

            Thorsten H. Ecke, Natalya Benderska-Söder, Ekkehardt Bismarck, Bas W.G. van Rhijn, Tilman Todenhöfer, and Bernd J. Schmitz-Dräger

 

13. Simplex Droplet Digital PCR Assays for the Detection of TERT Promoter Mutations in Urine Samples for the Non-Invasive Diagnosis of Urothelial Cancer

            Maria Zvereva, Md Ismail Hosen, Nathalie Forey, Mahdi Sheikh, Caroline Kannengiesser, Ibrahima Ba, Arnaud Manel, Emmanuel Vian, and Florence Le Calvez-Kelm

 

14. Predictive Biomarkers of Response to Neoadjuvant Therapy in Muscle Invasive Bladder Cancer

            Jussi Nikkola and Peter Black

 

15. Assessment of PD-L1 Status in Urothelial Cancer

            Veronika Bahlinger, Arndt Hartmann, and Markus Eckstein

 

Part V: Therapy Development

 

16. Epigenetic Priming and Development of New Combination Therapy Approaches

            Sarah Meneceur, Camilla M. Grunewald, Günter Niegisch, and Michèle. J. Hoffmann

 

17. Evaluation of FGFR Alteration Status in Urothelial Tumors

            Veronika Bahlinger, Markus Eckstein, Arndt Hartmann, and Robert Stöhr

 

18. Antibody-Drug-Conjugates (ADC): A Novel Treatment Option in Urothelial Carcinoma

            Günter Niegisch

 

19. Intravesical Infusion of Oncolytic Virus CG0070 in the Treatment of Bladder Cancer

            Paola Grandi, Andrea Darilek, Anay Moscu, Anu Pradhan, and Roger Li

 

20. Analysis of ICAM-1 Expression on Bladder Carcinoma Cell Lines and Infectivity and Oncolysis by Coxsackie Virus A21

            Kate Relph, Mehreen Arif, Hardev Pandha, Nicola Annels, and Guy R. Simpson