The Cytoskeleton: Imaging, Isolation, and Interaction

ISBN-13: 9781493959372 / Angielski / Miękka / 2016 / 359 str.

Rolf Dermietzel

Opis

This informative Neuromethods series book surveys the cytoskeleton, and offers detailed protocols on emerging techniques including in vivo and in vitro imaging and preparative strategies for isolation of cytoskeletal components of the central nervous system.

Spis treści

Part I: Imaging

 

1. Fluorescence Proteins and Time-Lapse Imaging of the Cytoskeleton

            Carsten Theiss and Karl Meller

 

2. Visualizing In Vitro Trafficking

            John Murray

 

Part II: Interaction, Dynamics, and Proteomics

 

3. Interaction of the Glycocalyx with the Actin Cytoskeleton

            Mia M. Thi, Eno E. Ebong, David C. Spray, and Sylvia O. Suadicani

 

4. Cytoskeleton Dynamics and Binding Factors

            Elena E. Grintsevich and Emil Reisler

 

5. Principles of Proteomic Approaches to the Cytoskeleton

            Christina Loosse, Katrin Marcus, and Bodo Schoenebeck

 

Part III: The Neuronal Cytoskeleton

 

6. Analyzing the Cytoskeletal Transcriptome: Sex Differences in Rat Hypothalamus

            Sanda Iacobas, Genevieve Neal-Perry, and Dumitru Andrei Iacobas

 

7. Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes

            Cantas Alev, Georg Zoidl, and Rolf Dermietzel

 

8. The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases

            Saar Oz and Illana Gozes

 

9. Neurofilaments: Properties, Functions, and Regulation

            Rodolphe Perrot and Joel Eyer

 

10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics 

            Micha E. Spira and Hadas Erez

 

11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton

            Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken

 

Part IV: Astroglia and the Cytoskeleton

 

12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact

            Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel

 

13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis

            Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson

 

Part V: Oligodendroglia and Myelin

 

14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System

            Christiane Richter-Landsberg

 

15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

            Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner

            Sanda Iacobas, Genevieve Neal-Perry, and Dumitru Andrei Iacobas

 

7. Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes

            Cantas Alev, Georg Zoidl, and Rolf Dermietzel

 

8. The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases

            Saar Oz and Illana Gozes

 

9. Neurofilaments: Properties, Functions, and Regulation

            Rodolphe Perrot and Joel Eyer

 

10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics 

            Micha E. Spira and Hadas Erez

 

11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton

            Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken

 

Part IV: Astroglia and the Cytoskeleton

 

12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact

            Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel

 

13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis

            Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson

 

Part V: Oligodendroglia and Myelin

 

14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System

            Christiane Richter-Landsberg

 

15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

            Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner

            Saar Oz and Illana Gozes

 

9. Neurofilaments: Properties, Functions, and Regulation

            Rodolphe Perrot and Joel Eyer

 

10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics 

            Micha E. Spira and Hadas Erez

 

11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton

            Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken

 

Part IV: Astroglia and the Cytoskeleton

 

12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact

            Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel

 

13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis

            Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson

 

Part V: Oligodendroglia and Myelin

 

14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System

            Christiane Richter-Landsberg

 

15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

            Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner

 

11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton

            Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken

 

Part IV: Astroglia and the Cytoskeleton

 

12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact

            Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel

 

13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis

            Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson

 

Part V: Oligodendroglia and Myelin

 

14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System

            Christiane Richter-Landsberg

 

15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

            Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner

            Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel

 

13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis

            Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson

 

Part V: Oligodendroglia and Myelin

 

14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System

            Christiane Richter-Landsberg

 

15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

            Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner

 

15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

            Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner

            Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner

Blurb

The cerebral cytoskeleton, considered to be the working horse in brain tissues, becomes particularly important when degenerative diseases of the nervous system are considered.  The Cytoskeleton: Imaging, Isolation, and Interaction assembles chapters on general aspects of the cytoskeleton, which are helpful for polishing knowledge of the cytoskeleton in a brief and informative way, and these pieces are flanked by a collection of detailed protocols on diverse emerging techniques including in vivo and in vitro imaging of the cytoskeleton at work as well as proteomics methodologies addressing preparative strategies for the isolation of cytoskeletal components of the central nervous system.  Written for the popular Neuromethods series, this work contains the kind of meticulous descriptions and implementation advice to guarantee successful results.

 

Authoritative and cutting-edge, The Cytoskeleton: Imaging, Isolation, and Interaction provides a compilation of papers written by a team of experts which aims to strengthen our efforts at overcoming at least some of the fatal outcomes of the diseased brain cytoskeleton.