Th9 Cells: Methods and Protocols

ISBN-13: 9781493983261 / Angielski / Miękka / 2018 / 259 str.

Ritobrata Goswami

Spis treści

Preface…
Table of Contents…
Contributing Authors..

1. Th9 Cells: New Member of T Helper Cell Family
Ritobrata Goswami

2. IL-9: Function, Sources, and Detection
Wilmer Gerardo Rojas-Zuleta and Elizabeth Sanchez

3. IL-9 Signaling Pathway: An Update
Dijendra Nath Roy and Ritobrata Goswami

4. A Method to In Vitro Differentiate Th9 Cells from Mouse Naïve CD4+ T Cells
Duy Pham

5. T Cell Receptor and Co-Stimulatory Signals for Th9 Generation
Françoise Meylan and Julio Gomez-Rodriguez

6. Polarizing Cytokines for Human Th9 Cell Differentiation
Prabhakar Putheti

7. Determining the Frequencies of Th9 Cells from Whole Blood
Anuradha Rajamanickam and Subash Babu

8. IL-9 Production by Nonconventional T Helper Cells
Silvia C. P. Almeida and Luis Graca

9. Prediction and Validation of Transcription Factors Binding Sites in the IL-9 Locus
William Orent and Wassim Elyaman

10. Flow Cytometric Assessment of STAT Molecules in Th9 Cells
Lucien P. Garo, Vanessa Beynon, and Gopal Murugaiyan

11. Transcription Factors Downstream of IL-4 and TGF- β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
Atsushi Sugimoto, Ryoji Kawakami, and Norihisa Mikami

12. Retroviral Transduction and Reporter Assay: Transcription Factor Co-Operation in Th9 Cell Development
Rukhsana Jabeen

13. Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid-Luminescent DNA-Precipitation Assay
Etienne Humblin, François Ghiringhello, and Frédérique Vegran

14. Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation
Alla Skapenko and Hendrik Schulze-Koops

15. Allergic Inflammation and Atopic Disease: Role of Th9 Cells
Pornpimon Angkasekwinai

16. Characterization of T Helper 9 (Th9) Cells in the Development of EAE and IBD
Sakshi Malik, Valerie Dardalhon, and Amit Awasthi

17. B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer
Alka Dwivedi, Sushant Kumar, and Rahul Purwar

18. Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models
Miguel Enrique Serrano Pino and Paula Licona-Limón

19. Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings
Patricia Keating and James X. Hartmann

Blurb

This volume presents comprehensive laboratory protocols that have been used to generate Th9 cells, both in vitro and in vivo. The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Th9 Cells: Methods and Protocols is a valuable resource for scientists in this field and will provide them with techniques to generate Th9 cells for specific downstream events.