Suicide Gene Therapy: Methods and Protocols

ISBN-13: 9781493989218 / Angielski / Twarda / 2018 / 190 str.

Düzgüneş, Nejat

Opis

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

Spis treści

1. Origins of Suicide Gene Therapy

            Nejat Düzgüneş

 

2. Cancer Suicide Gene Therapy with TK.007

            Jubayer A. Hossain, Kristoffer Riecken, Hrvoje Miletic, and Boris Fehse

 

3. Osteonectin Promoter-Mediated Suicide Gene Therapy of Prostate Cancer

            Wan-Chi Hsiao, Shian-Ying Sung, Leland W.K. Chung, and Chia-Ling Hsieh

 

4. Suicide Gene Therapy for Oral Squamous Cell Carcinoma

            Henrique Faneca, Nejat Düzgüneş, and Maria C. Pedroso de Lima

 

5. Generation of Suicide Gene-Modified Chimeric Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy

            Kentaro Minagawa, Mustafa Al-Obaidi, and Antonio Di Stasi

 

6. Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer

            Cestmir Altaner and Ursula Altanerova

 

7. Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy

            Erdogan Pekcan Erkan, Nurten Saydam, and Okay Saydam

 

8. Production of Viral Vectors with Suicide Genes by Utilizing the Intron-Splicing Mechanism of Insect Cells

            Haifeng Chen

 

9. Resveratrol-Responsive CArG Elements from the Egr-1 Promoter for the Induction of GADD45α to Arrest the G2/M Transition

            Qiwen Shi and Deepak Bhatia

 

10. Non-Invasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging

            Bryan Holvoet, Cindy Leten, Christophe M. Deroose, and Uwe Himmelreich

 

11. In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector

            Alexey A. Komissarov, Sergey V. Kostrov, and Ilya V. Demidyuk

 

12. Engineered Thymidine-Active Deoxycytidine Kinase for Bystander Killing of Malignant Cells

            Anton Neschadim and Jeffrey A. Medin

 

13. Photochemical Internalization Enhanced Non-Viral Suicide Gene Therapy

            Chung-Ho Sun, Kristian Berg, and Henry Hirschberg

 

14. Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro

            Nejat Düzgüneş, Jennifer Cheung, and Krystyna Konopka