Stem Cell Banking: Concepts and Protocols

ISBN-13: 9781493969197 / Angielski / Twarda / 2017 / 210 str.

Jeremy M. Crook; Tenneille Ludwig

Spis treści

Preface…
Table of Contents…
Contributing Authors…

Part I  Generic Themes in Stem Cell Banking 

1. Stem Cell Banking: A Global View

Glyn Stacey

2. Quality Assurance in Stem Cell Banking: Emphasis on Embryonic and Induced Pluripotent Stem Cell Banking

Therése Kallur, Pontus Blomberg, Sonya Stenfelt, Kristian Tryggvason, and Outi Hovatta

3. Acquisition and Reception of Primary Tissues, Cells, or Other Biological Specimens

Lyn E. Healy

4. Information Management

Alberto Labarga, Izaskun Beloqui, and Angel G. Martin

5. Cryopreservation: Vitrification and Control Rate Cooling

Charles J. Hunt

6. Quality Assured Characterization of Stem Cells for Safety in Banking for Clinical Application

Kevin Bruce, John D.M. Campbell, and Paul De Sousa

7. Ethics and Governance of Stem Cell Banks

Don Chalmers, Peter Rathjen, Joy Rathjen, and Dianne Nicol

Part II  Protocols for Pluripotent Stem Cell Banking

8. Derivation of Human Embryonic Stem Cells

Jeremy M. Crook, Lucy Kravets, Teija Peura, and Meri T. Firpo

9. Derivation of Human Induced Pluripotent Stem Cells in Chemically Defined Medium

Guokai Chen and Mahendra Rao

10. Culture, Adaptation, and Expansion of Pluripotent Stem Cells

Jennifer L. Brehm and Tenneille E. Ludwig

11. Cryobanking Pluripotent Stem Cells

Jeremy M. Crook, Eva Tomaskovic-Crook, and Tenneille E. Ludwig

12. Genome Editing in Human Pluripotent Stem Cells

Jared Carlson-Stevermer and Krishanu Saha

Part III  Protocols for Mesenchymal Stem Cell Banking

13.  Isolation, Culture, and Expansion of Mesenchymal Stem Cells

Izaskun Ferrin, Izaskun Beloqui, Lorea Zabaleta, Juan M. Salcedo, Cesar Trigueros, and Angel G. Martin

14.  Cryobanking Mesenchymal Stem Cells

Andrés Pavón, Izaskun Beloqui, Juan M. Salcedo, and Angel G. Martin

Part IV  Protocols for Human Neural Stem Cell Banking

15.  Culturing and Cryobanking Human Neural Stem Cells

Jeremy M. Crook and Eva Tomaskovic-Crook

Blurb

This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of “seed stock,” standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.