Spatial Pattern of Transcription Factor Binding Sites in genome

ISBN-13: 9783330345843 / Angielski / Miękka / 2017 / 60 str.

Divya Verma

Opis

A mammalian body consists of more than 200 types of cells; almost all of them having the same genome but different morphology and functions. These differences are due to cell-specific gene regulatory machinery. Mammalian genome has a complex organisation of regulatory sequences associated with different regulatory elements. The linear DNA in the nucleus is folded in a very complex manner and this folding brings distantly placed regions closer to each other. The combination of different DNA binding proteins such as transcription factors and histones; epigenetic marks such as gene position and dynamic modification of DNA and histones; and the three dimensional organisation of regulatory landscape interact in a pervasive and cell specific manner to generate complex spatiotemporal expression patterns. Chromatin Immunoprecipitation (ChIP seq) and Chromosome Conformation Capture (Hi C and 5C) approaches can be integrated with the whole transcriptome shotgun sequencing (RNA-Seq) to decipher Spatial Pattern of binding of regulatory elements to their target genes.

O autorze

Divya Verma is an Assistant Professor in the Department of Botany at Kalindi College, University of Delhi with an experience of twelve years of teaching. She completed her M.Sc. and Ph.D. in Botany from the MDS University, Ajmer. In 2012, she moved to the University of Edinburgh, U.K. for another Masters in the field of Bioinformatics.